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DOI: 10.1055/s-0029-1234510
UPLC/TOF-MS methodology for the on-line identification of secondary metabolites in four Scleria species (Cyperaceae): S. striatonux, S. verrucosa, S. boivinii and S. naumaniana
The DCM extract of the rhizome of S.striatonux exhibited IC50/IC90 values of 0.664µg/mL/1.043µg/mL (chloroquine sensitive strain D6) and 0.671µg/mL/1.147µg/mL (chloroquine resistant strain W2) of Plasmodium falciparum. Bioassay guided fractionation afforded 4 new sesquiterpenes [1]. The activity was related to one endoperoxyl derivative. Three other species of Scleria, S. bovinii, S. verrucosa and S. naumaniana were tested for their antimalarial activity on P. falciparum. Results revealed activity of Scleria boivinii IC50 4.25µg/mL (chloroquine sensitive, NF54). Combining the separation efficiency of UPLC and high resolution of TOF-MS detector, an analytical method was optimized in order to compare the metabolite profiles of crude extracts from the four Scleria species and to track compounds presenting sesquiterpene base structures.
References: [1] Nyongbela, K.D. et al. (2009) Nat. Prod. Comm., in press.
[2] Mve-Mba, C.E et al. (1996)J. Ess. Oil Res. 8:59–61.