Planta Med 2009; 75 - PD18
DOI: 10.1055/s-0029-1234497

Phytochemical study and biological evaluation of the stem of Derris ferruginea Bentham

S Morel 1, A Landreau 1, M Litaudon 2, S Derbré 1, S Fournier 3, P Richomme 1
  • 1SONAS, IFR 149-UPRES-EA 921, UFR des Sciences Pharmaceutiques et d'Ingénierie de la Santé, 16 Bd Daviers, 49100 Université d'Angers, France
  • 2ICSN, CNRS, Bât. 27, Avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France
  • 3PIAM, UFR Sciences, 2, Bd Lavoisier, 49045 Angers cedex 01

The genus Derris Loureiro belongs to the tribe Milletiae of the Leguminosae. It includes about fifteen species widely found in the tropical areas of Africa and Asia [1]. These plants have been traditionally used over centuries as fish poisoning, insecticide and pesticide, particularly due to their large production of rotenone [2]. Biological activities of Derris species are various: cytotoxic, antibacterial, antifungal, and antioxidant [3,4,5]. Though major secondary metabolites found in the genus are known to be flavonoids, including prenylated flavanones, and isoflavonoids such as rotenoids, very few phytochemical informations were available on D. ferruginea [6].

Different crude extracts were obtained from the stem (2kg, Soxhlet apparatus). They were fractionated using successively MPLC, LC, FCPC® (Fast Centrifugal Partition Chromatography, Kromaton Angers), Sephadex LH-20® gel and finally purified on preparative HPLC. Chemical structures of the isolated compounds were elucidated using 1H and 13C NMR spectrum as well as Mass Spectrometry. Most of these compounds were identified as prenylated flavonoids (flavanones and isoflavonoids). Biological effects of these compounds will be reported here, particularly antimicrobial, antiparasitic activities and inhibition of the formation of AGEs (Advanced Glycation End Products involved in age- and diabetes-related chronic diseases).

Refrences: 1. The Angiosperm Phylogeny Group (2003) Bot. J. Linn. Soc. 141:399–436.

2. Moretti, C. et al. (1982)J. Ethnopharmacol. 6:139–160.

3. Cheenpracha, S. et al. (2007) Can. J. Chem. 85:1019–1022.

4. Khan, M.R. et al. (2006) Fitoterapia 77:327–330.

5. Wangensteen, H. et al. (2006) Fitoterapia, 77:290–295.

6. Subba, N.Y. et al. (1946) Proc. Indian Acad. Sci. 24:344–348.