Planta Med 2009; 75 - PC3
DOI: 10.1055/s-0029-1234462

Phenolic constituents of Crithmum maritimum and their radical scavenging activity

S Ngom 1, 3, L Breant 1, 3, C Antheaume 2, C Minker 1, A Leick 1, 3, J Muller 1, 3, N Mekideche 3, A Lobstein 1
  • 1Laboratory of Pharmacognosy, UMR-CNRS 7200, Faculty of Pharmacy, 67400 Illkirch, France
  • 2SCA-NMR, University of Strasbourg, Faculty of Pharmacy, 67400 Illkirch, France
  • 3BiotechMarine Z.I. BP 72, 22260 Pontrieux, France

Crithmum maritimum L. (Apiaceae) is a perennial aromatic plant of the European littoral, traditionally used as a tonic and spice herb [1].

The aim of the present study was to measure radical scavenging activity (RSA) of C. maritimum extracts obtained with different polarity solvents and then to isolate by bioguided fractionation the antioxidative compounds, using three different free radicals: 2,2-diphenyl-1-picryl-1-picrylhydrazyl radical (DPPH·), hydroxyl (HO·) and superoxide anion (O2 ·–).

Thirteen phenolic compounds were purified and identified using HPLC-UV combined with TLC and spectroscopic methods (NMR, MS). In addition to 5-O-caffeoylquinic acid (1), rutin (2) and hyperoside (3) also described in this species2, ten additional compounds were isolated and identified for the first time in aerial parts of this halophyte: 4-O-caffeoylquinic acid (4), 4,5-O-dicaffeoylquinic acid (5), 3,5-O-dicaffeoylquinic acid (6), 3,4-O-dicaffeoylquinic acid (7), caffeic acid (8), isoquercitrin (9), diosmetin (10), quercitrin (11), quercetin (12) and luteolin (13).

All these isolated polyphenols exhibited potent RSA against DPPH· radical (2µM < IC50<6µM), O2 ·– radical (1µM < IC50<10µM) and HO· radical (0.6µM < IC50<1µM), for the most actives.

References: [1]Özcan, M. et al. (2001) Nahrung 45:353–356.

[2] Katsouri, E. et al. J. Ess. Oil Res. 13:303–308.