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Semin Thromb Hemost 2009; 35(5): 488-496
DOI: 10.1055/s-0029-1234144
© Thieme Medical Publishers

Microparticles and Arterial Disease

Andrew Blann1 , Eduard Shantsila1 , Alena Shantsila1
  • 1Haemostasis, Thrombosis and Vascular Biology Unit, University of Birmingham Centre for Cardiovascular Sciences, City Hospital, Birmingham, United Kingdom
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Publikationsverlauf

Publikationsdatum:
08. September 2009 (online)

    Lizenzen und Reprints

ABSTRACT

Microparticles (MPs) are small (diameter <1 μm) fragments that seem likely to represent some form of physiology and/or pathophysiology of the originating cell, be it endothelial, platelet, or leukocyte. Increased numbers of MPs are found in various disease states, including cardiovascular disease, and a considerable weight of literature suggests a value in dissecting the various aspects of cell biology. A role in coagulation has been proposed because there is evidence that some MPs expose tissue factor. This article reviews the definitions, mechanisms of production, and links with pathophysiology of MPs in arterial disease, and thus whether or not they can contribute to improved patient care.

KEYWORDS

Microparticles - endothelial cells - platelets - leukocytes - flow cytometry

REFERENCES

  • 1 Davì G, Patrono C. Platelet activation and atherothrombosis.  N Engl J Med. 2007;  357(24) 2482-2494
    CrossrefPubMedGoogle Scholar
  • 2 Blann A D, Lip G Y. Virchow’s triad revisited: the importance of soluble coagulation factors, the endothelium, and platelets.  Thromb Res. 2001;  101(4) 321-327
    CrossrefPubMedGoogle Scholar
  • 3 Libby P. Inflammation in atherosclerosis.  Nature. 2002;  420(6917) 868-874
    CrossrefPubMedGoogle Scholar
  • 4 Abrams C S, Ellison N, Budzynski A Z, Shattil S J. Direct detection of activated platelets and platelet-derived microparticles in humans.  Blood. 1990;  75(1) 128-138
    PubMedGoogle Scholar
  • 5 Scharf R E, Tomer A, Marzec U M, Teirstein P S, Ruggeri Z M, Harker L A. Activation of platelets in blood perfusing angioplasty-damaged coronary arteries. Flow cytometric detection.  Arterioscler Thromb. 1992;  12(12) 1475-1487
    PubMedGoogle Scholar
  • 6 Dachary-Prigent J, Freyssinet J M, Pasquet J M, Carron J C, Nurden A T. Annexin V as a probe of aminophospholipid exposure and platelet membrane vesiculation: a flow cytometry study showing a role for free sulfhydryl groups.  Blood. 1993;  81(10) 2554-2565
    PubMedGoogle Scholar
  • 7 Satta N, Toti F, Feugeas O et al.. Monocyte vesiculation is a possible mechanism for dissemination of membrane-associated procoagulant activities and adhesion molecules after stimulation by lipopolysaccharide.  J Immunol. 1994;  153(7) 3245-3255
    PubMedGoogle Scholar
  • 8 Mallat Z, Hugel B, Ohan J, Lesèche G, Freyssinet J M, Tedgui A. Shed membrane microparticles with procoagulant potential in human atherosclerotic plaques: a role for apoptosis in plaque thrombogenicity.  Circulation. 1999;  99(3) 348-353
    CrossrefPubMedGoogle Scholar
  • 9 Combes V, Simon A C, Grau G E et al.. In vitro generation of endothelial microparticles and possible prothrombotic activity in patients with lupus anticoagulant.  J Clin Invest. 1999;  104(1) 93-102
    CrossrefPubMedGoogle Scholar
  • 10 ISTH Scientific Subcommittee Minutes .Papers presented at: 54th Annual Scientific and Standardization Committee Meeting; July 2–5, 2008; Vienna, Austria; and 55th Annual Scientific and Standardization Committee Meeting July 6–11, 2009 Boston, MA; Available at: http://www.med.unc.edu/isth/ssc/04sscminutes/04wg_vascular_biology.html
    PubMed
  • 11 Perez-Pujol S, Marker P H, Key N S. Platelet microparticles are heterogeneous and highly dependent on the activation mechanism: studies using a new digital flow cytometer.  Cytometry A. 2007;  71(1) 38-45
    PubMedGoogle Scholar
  • 12 Lin Y H, Lee G B. Optically induced flow cytometry for continuous microparticle counting and sorting.  Biosens Bioelectron. 2008;  24(4) 572-578
    CrossrefPubMedGoogle Scholar
  • 13 Macey M, Azam U, McCarthy D et al.. Evaluation of the anticoagulants EDTA and citrate, theophylline, adenosine, and dipyridamole (CTAD) for assessing platelet activation on the ADVIA 120 hematology system.  Clin Chem. 2002;  48(6 Pt 1) 891-899
    PubMedGoogle Scholar
  • 14 Jy W, Horstman L L, Jimenez J J et al.. Measuring circulating cell-derived microparticles.  J Thromb Haemost. 2004;  2 1842-1851
    CrossrefPubMedGoogle Scholar
  • 15 Shah M D, Bergeron A L, Dong J F, López J A. Flow cytometric measurement of microparticles: pitfalls and protocol modifications.  Platelets. 2008;  19(5) 365-372
    CrossrefPubMedGoogle Scholar
  • 16 Jimenez J J, Jy W, Mauro L M, Soderland C, Horstman L L, Ahn Y S. Endothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis.  Thromb Res. 2003;  109(4) 175-180
    CrossrefPubMedGoogle Scholar
  • 17 Banfi C, Brioschi M, Wait R et al.. Proteome of endothelial cell-derived procoagulant microparticles.  Proteomics. 2005;  5(17) 4443-4455
    CrossrefPubMedGoogle Scholar
  • 18 Koga H, Sugiyama S, Kugiyama K et al.. Elevated levels of VE-cadherin-positive endothelial microparticles in patients with type 2 diabetes mellitus and coronary artery disease.  J Am Coll Cardiol. 2005;  45(10) 1622-1630
    CrossrefPubMedGoogle Scholar
  • 19 Abid Hussein M N, Böing A N, Biró E et al.. Phospholipid composition of in vitro endothelial microparticles and their in vivo thrombogenic properties.  Thromb Res. 2008;  121(6) 865-871
    CrossrefPubMedGoogle Scholar
  • 20 Flaumenhaft R, Dilks J R, Richardson J et al.. Megakaryocyte-derived microparticles: direct visualization and distinction from platelet-derived microparticles.  Blood. 2009;  113(5) 1112-1121
    CrossrefPubMedGoogle Scholar
  • 21 George J N, Pickett E B, Saucerman S et al.. Platelet surface glycoproteins. Studies on resting and activated platelets and platelet membrane microparticles in normal subjects, and observations in patients during adult respiratory distress syndrome and cardiac surgery.  J Clin Invest. 1986;  78(2) 340-348
    CrossrefPubMedGoogle Scholar
  • 22 Garcia B A, Smalley D M, Cho H, Shabanowitz J, Ley K, Hunt D F. The platelet microparticle proteome.  J Proteome Res. 2005;  4(5) 1516-1521
    CrossrefPubMedGoogle Scholar
  • 23 Biró E, Akkerman J W, Hoek F J et al.. The phospholipid composition and cholesterol content of platelet-derived microparticles: a comparison with platelet membrane fractions.  J Thromb Haemost. 2005;  3(12) 2754-2763
    CrossrefPubMedGoogle Scholar
  • 24 Morel O, Toti F, Hugel B et al.. Procoagulant microparticles: disrupting the vascular homeostasis equation?.  Arterioscler Thromb Vasc Biol. 2006;  26(12) 2594-2604
    CrossrefPubMedGoogle Scholar
  • 25 Barry O P, Praticò D, Savani R C, FitzGerald G A. Modulation of monocyte-endothelial cell interactions by platelet microparticles.  J Clin Invest. 1998;  102(1) 136-144
    CrossrefPubMedGoogle Scholar
  • 26 Gambim M H, do Carmo AdeO, Marti L, Veríssimo-Filho S, Lopes L R, Janiszewski M. Platelet-derived exosomes induce endothelial cell apoptosis through peroxynitrite generation: experimental evidence for a novel mechanism of septic vascular dysfunction.  Crit Care. 2007;  11(5) R107
    CrossrefPubMedGoogle Scholar
  • 27 Jy W, Mao W W, Horstman L, Tao J, Ahn Y S. Platelet microparticles bind, activate and aggregate neutrophils in vitro.  Blood Cells Mol Dis. 1995;  21(3) 217-231, discussion 231a
    CrossrefPubMedGoogle Scholar
  • 28 Gasser O, Hess C, Miot S, Deon C, Sanchez J C, Schifferli J A. Characterisation and properties of ectosomes released by human polymorphonuclear neutrophils.  Exp Cell Res. 2003;  285(2) 243-257
    CrossrefPubMedGoogle Scholar
  • 29 Mesri M, Altieri D C. Endothelial cell activation by leukocyte microparticles.  J Immunol. 1998;  161(8) 4382-4387
    PubMedGoogle Scholar
  • 30 Pluskota E, Woody N M, Szpak D et al.. Expression, activation, and function of integrin alphaMbeta2 (Mac-1) on neutrophil-derived microparticles.  Blood. 2008;  112(6) 2327-2335
    CrossrefPubMedGoogle Scholar
  • 31 Scanu A, Molnarfi N, Brandt K J, Gruaz L, Dayer J M, Burger D. Stimulated T cells generate microparticles, which mimic cellular contact activation of human monocytes: differential regulation of pro- and anti-inflammatory cytokine production by high-density lipoproteins.  J Leukoc Biol. 2008;  83(4) 921-927
    CrossrefPubMedGoogle Scholar
  • 32 Berckmans R J, Neiuwland R, Böing A N, Romijn F P, Hack C E, Sturk A. Cell-derived microparticles circulate in healthy humans and support low grade thrombin generation.  Thromb Haemost. 2001;  85(4) 639-646
    PubMedGoogle Scholar
  • 33 Ueba T, Haze T, Sugiyama M et al.. Level, distribution and correlates of platelet-derived microparticles in healthy individuals with special reference to the metabolic syndrome.  Thromb Haemost. 2008;  100(2) 280-285
    PubMedGoogle Scholar
  • 34 Wang J M, Huang Y J, Wang Y et al.. Increased circulating CD31+/CD42-microparticles are associated with impaired systemic artery elasticity in healthy subjects.  Am J Hypertens. 2007;  20(9) 957-964
    CrossrefPubMedGoogle Scholar
  • 35 van der Zee P M, Biró E, Ko Y et al.. P-selectin- and CD63-exposing platelet microparticles reflect platelet activation in peripheral arterial disease and myocardial infarction.  Clin Chem. 2006;  52(4) 657-664
    CrossrefPubMedGoogle Scholar
  • 36 Michelson A D, Rajasekhar D, Bednarek F J, Barnard M R. Platelet and platelet-derived microparticle surface factor V/Va binding in whole blood: differences between neonates and adults.  Thromb Haemost. 2000;  84(4) 689-694
    PubMedGoogle Scholar
  • 37 Nomura S, Miyazaki Y, Miyake T et al.. Detection of platelet-derived microparticles in patients with diabetes.  Am J Hematol. 1993;  44(3) 213
    PubMedGoogle Scholar
  • 38 Nomura S, Suzuki M, Katsura K et al.. Platelet-derived microparticles may influence the development of atherosclerosis in diabetes mellitus.  Atherosclerosis. 1995;  116(2) 235-240
    CrossrefPubMedGoogle Scholar
  • 39 Tan K T, Tayebjee M H, Lim H S, Lip G Y. Clinically apparent atherosclerotic disease in diabetes is associated with an increase in platelet microparticle levels.  Diabet Med. 2005;  22(12) 1657-1662
    CrossrefPubMedGoogle Scholar
  • 40 Ogata N, Imaizumi M, Nomura S et al.. Increased levels of platelet-derived microparticles in patients with diabetic retinopathy.  Diabetes Res Clin Pract. 2005;  68(3) 193-201
    CrossrefPubMedGoogle Scholar
  • 41 Koga H, Sugiyama S, Kugiyama K et al.. Elevated levels of remnant lipoproteins are associated with plasma platelet microparticles in patients with type-2 diabetes mellitus without obstructive coronary artery disease.  Eur Heart J. 2006;  27(7) 817-823
    CrossrefPubMedGoogle Scholar
  • 42 Bernard S, Loffroy R, Sérusclat A et al.. Increased levels of endothelial microparticles CD144 (VE-Cadherin) positives in type 2 diabetic patients with coronary noncalcified plaques evaluated by multidetector computed tomography (MDCT).  Atherosclerosis. 2009;  203(2) 429-435
    CrossrefPubMedGoogle Scholar
  • 43 Ogata N, Nomura S, Shouzu A, Imaizumi M, Arichi M, Matsumura M. Elevation of monocyte-derived microparticles in patients with diabetic retinopathy.  Diabetes Res Clin Pract. 2006;  73(3) 241-248
    CrossrefPubMedGoogle Scholar
  • 44 Sabatier F, Darmon P, Hugel B et al.. Type 1 and type 2 diabetic patients display different patterns of cellular microparticles.  Diabetes. 2002;  51(9) 2840-2845
    CrossrefPubMedGoogle Scholar
  • 45 Preston R A, Jy W, Jimenez J J et al.. Effects of severe hypertension on endothelial and platelet microparticles.  Hypertension. 2003;  41(2) 211-217
    CrossrefPubMedGoogle Scholar
  • 46 Wang J M, Su C, Wang Y et al.. Elevated circulating endothelial microparticles and brachial-ankle pulse wave velocity in well-controlled hypertensive patients.  J Hum Hypertens. 2009;  23(5) 307-315
    CrossrefPubMedGoogle Scholar
  • 47 Bakouboula B, Morel O, Faure A et al.. Procoagulant membrane microparticles correlate with the severity of pulmonary arterial hypertension.  Am J Respir Crit Care Med. 2008;  177(5) 536-543
    CrossrefPubMedGoogle Scholar
  • 48 Amabile N, Heiss C, Real W M et al.. Circulating endothelial microparticle levels predict hemodynamic severity of pulmonary hypertension.  Am J Respir Crit Care Med. 2008;  177(11) 1268-1275
    CrossrefPubMedGoogle Scholar
  • 49 Casey R G, Joyce M, Roche-Nagle G, Cox D, Bouchier-Hayes D J. Young male smokers have altered platelets and endothelium that precedes atherosclerosis.  J Surg Res. 2004;  116(2) 227-233
    CrossrefPubMedGoogle Scholar
  • 50 Goichot B, Grunebaum L, Desprez D et al.. Circulating procoagulant microparticles in obesity.  Diabetes Metab. 2006;  32(1) 82-85
    CrossrefPubMedGoogle Scholar
  • 51 Esposito K, Ciotola M, Schisano B et al.. Endothelial microparticles correlate with endothelial dysfunction in obese women.  J Clin Endocrinol Metab. 2006;  91(9) 3676-3679
    CrossrefPubMedGoogle Scholar
  • 52 Bulut D, Maier K, Bulut-Streich N, Börgel J, Hanefeld C, Mügge A. Circulating endothelial microparticles correlate inversely with endothelial function in patients with ischemic left ventricular dysfunction.  J Card Fail. 2008;  14(4) 336-340
    CrossrefPubMedGoogle Scholar
  • 53 Werner N, Wassmann S, Ahlers P, Kosiol S, Nickenig G. Circulating CD31+/annexin V+ apoptotic microparticles correlate with coronary endothelial function in patients with coronary artery disease.  Arterioscler Thromb Vasc Biol. 2006;  26(1) 112-116
    CrossrefPubMedGoogle Scholar
  • 54 Héloire F, Weill B, Weber S, Batteux F. Aggregates of endothelial microparticles and platelets circulate in peripheral blood. Variations during stable coronary disease and acute myocardial infarction.  Thromb Res. 2003;  110(4) 173-180
    CrossrefPubMedGoogle Scholar
  • 55 Leroyer A S, Rautou P E, Silvestre J S et al.. CD40 ligand+ microparticles from human atherosclerotic plaques stimulate endothelial proliferation and angiogenesis a potential mechanism for intraplaque neovascularization.  J Am Coll Cardiol. 2008;  52(16) 1302-1311
    CrossrefPubMedGoogle Scholar
  • 56 Canault M, Leroyer A S, Peiretti F et al.. Microparticles of human atherosclerotic plaques enhance the shedding of the tumor necrosis factor-alpha converting enzyme/ADAM17 substrates, tumor necrosis factor and tumor necrosis factor receptor-1.  Am J Pathol. 2007;  171(5) 1713-1723
    CrossrefPubMedGoogle Scholar
  • 57 Craft J A, Marsh N A. Increased generation of platelet-derived microparticles following percutaneous transluminal coronary angioplasty.  Blood Coagul Fibrinolysis. 2003;  14(8) 719-728
    CrossrefPubMedGoogle Scholar
  • 58 Bernal-Mizrachi L, Jy W, Jimenez J J et al.. High levels of circulating endothelial microparticles in patients with acute coronary syndromes.  Am Heart J. 2003;  145(6) 962-970
    CrossrefPubMedGoogle Scholar
  • 59 Zielińska M, Koniarek W, Goch J H et al.. Circulating endothelial microparticles in patients with acute myocardial infarction.  Kardiol Pol. 2005;  62(6) 531-542, discussion 543–544
    PubMedGoogle Scholar
  • 60 Katopodis J N, Kolodny L, Jy W et al.. Platelet microparticles and calcium homeostasis in acute coronary ischemias.  Am J Hematol. 1997;  54(2) 95-101
    CrossrefPubMedGoogle Scholar
  • 61 Bernal-Mizrachi L, Jy W, Fierro C et al.. Endothelial microparticles correlate with high-risk angiographic lesions in acute coronary syndromes.  Int J Cardiol. 2004;  97(3) 439-446
    CrossrefPubMedGoogle Scholar
  • 62 Huisse M G, Lanoy E, Tcheche D et al.. Prothrombotic markers and early spontaneous recanalization in ST-segment elevation myocardial infarction.  Thromb Haemost. 2007;  98(2) 420-426
    PubMedGoogle Scholar
  • 63 Matsumoto N, Nomura S, Kamihata H, Kimura Y, Iwasaka T. Association of platelet-derived microparticles with C-C chemokines on vascular complication in patients with acute myocardial infarction.  Clin Appl Thromb Hemost. 2002;  8(3) 279-286
    CrossrefPubMedGoogle Scholar
  • 64 Singh N, Gemmell C H, Daly P A, Yeo E L. Elevated platelet-derived microparticle levels during unstable angina.  Can J Cardiol. 1995;  11(11) 1015-1021
    PubMedGoogle Scholar
  • 65 Michelsen A E, Brodin E, Brosstad F, Hansen J B. Increased level of platelet microparticles in survivors of myocardial infarction.  Scand J Clin Lab Invest. 2008;  68(5) 386-392
    CrossrefPubMedGoogle Scholar
  • 66 Gawaz M, Neumann F J, Ott I, Schiessler A, Schömig A. Platelet function in acute myocardial infarction treated with direct angioplasty.  Circulation. 1996;  93(2) 229-237
    CrossrefPubMedGoogle Scholar
  • 67 Simak J, Gelderman M P, Yu H, Wright V, Baird A E. Circulating endothelial microparticles in acute ischemic stroke: a link to severity, lesion volume and outcome.  J Thromb Haemost. 2006;  4(6) 1296-1302
    CrossrefPubMedGoogle Scholar
  • 68 Williams J B, Jauch E C, Lindsell C J, Campos B. Endothelial microparticle levels are similar in acute ischemic stroke and stroke mimics due to activation and not apoptosis/necrosis.  Acad Emerg Med. 2007;  14(8) 685-690
    CrossrefPubMedGoogle Scholar
  • 69 Lee Y J, Jy W, Horstman L L et al.. Elevated platelet microparticles in transient ischemic attacks, lacunar infarcts, and multiinfarct dementias.  Thromb Res. 1993;  72(4) 295-304
    CrossrefPubMedGoogle Scholar
  • 70 Geiser T, Sturzenegger M, Genewein U, Haeberli A, Beer J H. Mechanisms of cerebrovascular events as assessed by procoagulant activity, cerebral microemboli, and platelet microparticles in patients with prosthetic heart valves.  Stroke. 1998;  29(9) 1770-1777
    CrossrefPubMedGoogle Scholar
  • 71 Zeiger F, Stephan S, Hoheisel G, Pfeiffer D, Ruehlmann C, Koksch M. P-Selectin expression, platelet aggregates, and platelet-derived microparticle formation are increased in peripheral arterial disease.  Blood Coagul Fibrinolysis. 2000;  11(8) 723-728
    CrossrefPubMedGoogle Scholar
  • 72 Nomura S, Imamura A, Okuno M et al.. Platelet-derived microparticles in patients with arteriosclerosis obliterans: enhancement of high shear-induced microparticle generation by cytokines.  Thromb Res. 2000;  98(4) 257-268
    CrossrefPubMedGoogle Scholar
  • 73 Lechner D, Weltermann A. Circulating tissue factor-exposing microparticles.  Thromb Res. 2008;  122(suppl 1) S47-S54
    CrossrefPubMedGoogle Scholar
  • 74 Tans G, Rosing J, Thomassen M C, Heeb M J, Zwaal R F, Griffin J H. Comparison of anticoagulant and procoagulant activities of stimulated platelets and platelet-derived microparticles.  Blood. 1991;  77(12) 2641-2648
    PubMedGoogle Scholar
  • 75 Sinauridze E I, Kireev D A, Popenko N Y et al.. Platelet microparticle membranes have 50- to 100-fold higher specific procoagulant activity than activated platelets.  Thromb Haemost. 2007;  97(3) 425-434
    Artikel in Thieme ConnectPubMedGoogle Scholar
  • 76 Ilveskero S, Siljander P, Lassila R. Procoagulant activity on platelets adhered to collagen or plasma clot.  Arterioscler Thromb Vasc Biol. 2001;  21(4) 628-635
    CrossrefPubMedGoogle Scholar
  • 77 Siljander P, Carpen O, Lassila R. Platelet-derived microparticles associate with fibrin during thrombosis.  Blood. 1996;  87(11) 4651-4663
    CrossrefPubMedGoogle Scholar
  • 78 Hoffman M, Monroe D M, Roberts H R. Coagulation factor IXa binding to activated platelets and platelet-derived microparticles: a flow cytometric study.  Thromb Haemost. 1992;  68(1) 74-78
    PubMedGoogle Scholar
  • 79 Falati S, Liu Q, Gross P et al.. Accumulation of tissue factor into developing thrombi in vivo is dependent upon microparticle P-selectin glycoprotein ligand 1 and platelet P-selectin.  J Exp Med. 2003;  197(11) 1585-1598
    CrossrefPubMedGoogle Scholar
  • 80 Poitevin S, Cochery-Nouvellon E, Dupont A, Nguyen P. Monocyte IL-10 produced in response to lipopolysaccharide modulates thrombin generation by inhibiting tissue factor expression and release of active tissue factor-bound microparticles.  Thromb Haemost. 2007;  97(4) 598-607
    PubMedGoogle Scholar
  • 81 Rauch U, Bonderman D, Bohrmann B et al.. Transfer of tissue factor from leukocytes to platelets is mediated by CD15 and tissue factor.  Blood. 2000;  96(1) 170-175
    PubMedGoogle Scholar
  • 82 Kushak R I, Nestoridi E, Lambert J, Selig M K, Ingelfinger J R, Grabowski E F. Detached endothelial cells and microparticles as sources of tissue factor activity.  Thromb Res. 2005;  116(5) 409-419
    CrossrefPubMedGoogle Scholar
  • 83 Jy W, Jimenez J J, Mauro L M et al.. Endothelial microparticles induce formation of platelet aggregates via a von Willebrand factor/ristocetin dependent pathway, rendering them resistant to dissociation.  J Thromb Haemost. 2005;  3(6) 1301-1308
    CrossrefPubMedGoogle Scholar
  • 84 Brodsky S V, Malinowski K, Golightly M, Jesty J, Goligorsky M S. Plasminogen activator inhibitor-1 promotes formation of endothelial microparticles with procoagulant potential.  Circulation. 2002;  106(18) 2372-2378
    CrossrefPubMedGoogle Scholar
  • 85 Leroyer A S, Isobe H, Lesèche G et al.. Cellular origins and thrombogenic activity of microparticles isolated from human atherosclerotic plaques.  J Am Coll Cardiol. 2007;  49(7) 772-777
    CrossrefPubMedGoogle Scholar
  • 86 Chironi G, Simon A, Hugel B et al.. Circulating leukocyte-derived microparticles predict subclinical atherosclerosis burden in asymptomatic subjects.  Arterioscler Thromb Vasc Biol. 2006;  26(12) 2775-2780
    CrossrefPubMedGoogle Scholar
  • 87 Namba M, Tanaka A, Shimada K et al.. Circulating platelet-derived microparticles are associated with atherothrombotic events: a marker for vulnerable blood.  Arterioscler Thromb Vasc Biol. 2007;  27(1) 255-256
    CrossrefPubMedGoogle Scholar
  • 88 Del Turco S, Basta G, Lazzerini G et al.. Effect of the administration of n-3 polyunsaturated fatty acids on circulating levels of microparticles in patients with a previous myocardial infarction.  Haematologica. 2008;  93(6) 892-899
    CrossrefPubMedGoogle Scholar
  • 89 Matzdorff A C, Kühnel G, Kemkes-Matthes B, Pralle H, Voss R, Fareed J. Effect of glycoprotein IIb/IIIa inhibitors on CD62p expression, platelet aggregates, and microparticles in vitro.  J Lab Clin Med. 2000;  135(3) 247-255
    CrossrefPubMedGoogle Scholar
  • 90 Nomura S, Shouzu A, Omoto S, Nishikawa M, Fukuhara S, Iwasaka T. Effect of valsartan on monocyte/endothelial cell activation markers and adiponectin in hypertensive patients with type 2 diabetes mellitus.  Thromb Res. 2006;  117(4) 385-392
    CrossrefPubMedGoogle Scholar
  • 91 Serebruany V L, Malinin A I, Jerome S D et al.. Effects of clopidogrel and aspirin combination versus aspirin alone on platelet aggregation and major receptor expression in patients with heart failure: the Plavix Use for Treatment Of Congestive Heart Failure (PLUTO-CHF) trial.  Am Heart J. 2003;  146 713-720
    CrossrefPubMedGoogle Scholar
  • 92 Nomura S, Takahashi N, Inami N et al.. Probucol and ticlopidine: effect on platelet and monocyte activation markers in hyperlipidemic patients with and without type 2 diabetes.  Atherosclerosis. 2004;  174(2) 329-335
    PubMedGoogle Scholar
  • 93 Diamant M, Tushuizen M E, Abid-Hussein M N et al.. Simvastatin-induced endothelial cell detachment and microparticle release are prenylation dependent.  Thromb Haemost. 2008;  100(3) 489-497
    PubMedGoogle Scholar
  • 94 Esposito K, Ciotola M, Giugliano D. Pioglitazone reduces endothelial microparticles in the metabolic syndrome.  Arterioscler Thromb Vasc Biol. 2006;  26(8) 1926
    CrossrefPubMedGoogle Scholar
  • 95 Pirro M, Schillaci G, Bagaglia F et al.. Microparticles derived from endothelial progenitor cells in patients at different cardiovascular risk.  Atherosclerosis. 2008;  197(2) 757-767
    CrossrefPubMedGoogle Scholar
  • 96 Pirro M, Schillaci G, Paltriccia R et al.. Increased ratio of CD31 + /CD42- microparticles to endothelial progenitors as a novel marker of atherosclerosis in hypercholesterolemia.  Arterioscler Thromb Vasc Biol. 2006;  26(11) 2530-2535
    CrossrefPubMedGoogle Scholar
  • 97 Lacroix R, Sabatier F, Mialhe A et al.. Activation of plasminogen into plasmin at the surface of endothelial microparticles: a mechanism that modulates angiogenic properties of endothelial progenitor cells in vitro.  Blood. 2007;  110(7) 2432-2439
    CrossrefPubMedGoogle Scholar
  • 98 George F D. Microparticles in vascular diseases.  Thromb Res. 2008;  122(Suppl 1) S55-S59
    CrossrefPubMedGoogle Scholar
  • 99 Robert S, Poncelet P, Lacroix R et al.. Standardization of platelet-derived microparticle counting using calibrated beads and a Cytomics FC500 routine flow cytometer: a first step towards multicenter studies?.  J Thromb Haemost. 2009;  7(1) 190-197
    CrossrefPubMedGoogle Scholar
  • 100 Shet A S. Characterizing blood microparticles: technical aspects and challenges.  Vasc Health Risk Manag. 2008;  4(4) 769-774
    PubMedGoogle Scholar

Dr. A.D. Blann

Haemostasis, Thrombosis and Vascular Biology Unit, University of Birmingham Centre for Cardiovascular Sciences

City Hospital, Birmingham B18 7QH, UK

eMail: a.blann@bham.ac.uk

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