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Exp Clin Endocrinol Diabetes 1990; 96(5): 129-137
DOI: 10.1055/s-0029-1211001
DOI: 10.1055/s-0029-1211001
Original
© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York
Radioimmunoassay of Corticotropin - Releasing Hormone
Weitere Informationen
Publikationsverlauf
1990
Publikationsdatum:
16. Juli 2009 (online)
Summary
A specific double antibody radioimmunoassay (RIA) for human-Corticotropin-Releasing Hormone (hCRF) using an antibody to synthetic hCRF was established. This antibody allowed a usable range of 10 pg to 5 ng of CRF per ml in the assay.
Comparing the efficiency of various plasma extraction procedures, the extraction with ice-cold methanol was found to be the most simple and rapid method with an extraction efficiency of more than 80%.
The reliability of the radioimmunoassay was shown under physiological and pathophysiological conditions.
Key words
CRF - Radioimmunoassay - Plasma extraction - Normal and pathological values