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DOI: 10.1055/s-0029-1210919
© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York
In Vitro Secretion of Hormones from the Islets of Pancreas with Acinar Atrophy — Influence of Digestion with Collagenase
1) Recipient of an Alexander von Humboldt fellowship.Publication History
1988
Publication Date:
16 July 2009 (online)
Summary
The release of insulin, glucagon and somatostatin from islets isolated by micro-dissection, and islets isolated by aid of different duration of collagenase digestion from pancreases with exocrine atrophy was assessed. Prior collagenase digestion caused an increased release of insulin and glucagon, but not somatostatin; also, the non-suppressibility of glucagon secretion despite high glucose concentration in the medium was characteristic for those islets. Additional data suggest that in the absence of intrinsic pancreatic proteases the nature of the damage conferred by collagenase to islet B- and A-cells is different. In the light of action of epinephrine, an inhibitor of insulin secretion, possible mechanisms responsible for disturbed hormone release in the presence of proteolytic enzymes are discussed.
Key Words
Isolated islets - Pancreas with exocrine atrophy - Collagenase - Secretion of islets hormones