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DOI: 10.1055/s-0029-1210801
© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York
Solubilization of Rat Kidney Plasma Membrane Proteins Associated with 3H-Aldosterone
Publikationsverlauf
1988
Publikationsdatum:
16. Juli 2009 (online)
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Summary
The treatment of rat kidney plasma membranes with sodium dodecyl sulphate (SDS) did not essentially affect the ability of the membranes for 3H-aldosterone binding as compared with the intact plasma membranes (Ozegovic et al., 1977). A gel filtration of 3H-aldosterone — kidney plasma membranes complex on Sepharose 6B yielded 2 protein and 2 3H-aldosterone peaks. The proteins which were eluted in the first peak were associated with the first 3H-aldosterone peak while the second 3H-aldosterone peak was eluted with Ve corresponding to Ve of free 3H-aldosterone.
Spironolactone, a competitive antagonist of aldosterone, prevented the binding of 3H-aldosterone to the membrane proteins.
The results demonstrated a high affinity of the kidney plasma membranes solubilized with SDS and a specificity of aldosterone binding to the plasma membrane proteins of higher molecular mass.
Key words
Aldosterone - Kidney - Plasma membrane proteins