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Exp Clin Endocrinol Diabetes 1987; 89(3): 283-289
DOI: 10.1055/s-0029-1210651
Original

© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

In Vitro and in Vivo Behaviour of Rat Islets of Langerhans Treated with MHC Antisera and Complement2)

Beate Kuttler, S. Schmidt, Ingrid Klöting, O. Stark1
  • Central Institute of Diabetes “Gerhardt Katsch”, Department of Experimental Reseach, Karlsburg/GDR
  • 1Institute of Biology, Charles-University, Prague/Czechoslovakia
2) This study was part of the research project HFR M22 of the Ministry of Health of the GDR.
Further Information

Publication History

1986

Publication Date:
16 July 2009 (online)

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Summary

The in vitro and in vivo behaviour of rat islets of Langerhans pretreated with polyclonal MHC antisera and complement was investigated. Complement-dependent cytotoxicity against rat islets was mediated by the antisera which were either directed against all MHC products (“a anti-u”) or against products of the B-region of rat MHC (“ Ba anti-Bu”). There were only minor alterations in glucose-stimulated insulin secretion and in 3H-leucine incoroporation into islet proteins and into (pro) insulin in the preteated islets. The syngeneic transplantion of “Ba anti-Bu” - treated islets led to a permanent graft survival in all streptozotocin-diabetic rats but after “a anti-u”- pretreatment permanent graft survival was only achieved in 33% of the syngeneic recipients. It is concluded, that in the latter case a complement-dependent cytotoxicity is exerted against the islets which may not allow sufficient in vivo survival. Now it appears necessary to look for effects of pretreatment prior to an allogeneic transplantation.

Key words

MHC antisera - Complement-dependent cytotoxicity - Beta-cell function - Syngeneic islet transplantion

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