Oestradiol and Testosterone Binding Sites in Mice Tibiae and Their Relationship with Bone Growth

Summary

High affinity oestradiol and testosterone binding sites were found in tibiae cytosol from entire male and female of different ages. Scatchard assay allowed to estimate a Kd of 2.7 × 10⁻⁹ M for oestradiol binding sites indicating that the ³H-oestradiol binding was of high affinity.

Oestradiol and testosterone binding sites abundance in mice tibiae are subject to change with age. It is not easy to establish a direct correlation between these changes and the values reported here on bone growth in weight and length, however seems possible to point a negative relationship between bone lengthening and oestradiol binding site levels in female, as well a positive relationship with testosterone in both sexes.

The presence of oestradiol and testosterone binding sites in epiphyses and not in the diaphyses reinforces the hypothesis that both are playing some role in bone growth.

Key words

Bone growth - Oestradiol - Testosterone receptors