Planta Med 2009; 75(11): 1209-1214
DOI: 10.1055/s-0029-1185540
Pharmacology
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Effects of 2,3,4′,5-Tetrahydroxystilbene 2-O-β-D-Glucoside on Vascular Endothelial Dysfunction in Atherogenic-Diet Rats

Wei Zhang1 , Xiao-Le Xu1 , Yu-Qin Wang1 , Chun-Hua Wang1 , Wei-Zhong Zhu1
  • 1Department of Pharmacology, School of Medicine, Nantong University, Nantong, P. R. China
Further Information

Publication History

received Nov. 25, 2008 revised February 19, 2009

accepted February 20, 2009

Publication Date:
06 April 2009 (online)

Abstract

2,3,4′,5-Tetrahydroxystilbene 2-O-β-D-glucoside (TSG), an active component extracted from Polygonum multiflorum, has been found to have an anti-atherosclerotic effect. The aim of this study was to investigate whether the TSG could prevent the development of atherosclerosis through influencing endothelial function in atherogenic-diet rats and to explore the possible mechanisms. Vascular endothelial dysfunction was assessed using isolated aortic ring preparation, transmission electron microscopy of the aorta, and levels of nitrate/nitrite (NOx) in serum and aorta. Endothelial nitric oxide (NO) synthase (eNOS) and inducible NO synthase (iNOS) mRNA and protein expression were also measured. After 12 weeks treatment, TSG improved acetylcholine-induced endothelium-dependent relaxation, prevented intimal remodeling, inhibited the decreased NOx content in serum and aorta in atherogenic-diet rats. Furthermore, the observed decreased eNOS mRNA and protein expression and increased iNOS mRNA and protein expression in atherogenic-diet rats were attenuated by TSG treatment. These results suggest that TSG could restore vascular endothelial function, which may be related to its ability to prevent changes of eNOS and iNOS expression, leading to preservation of NO bioactivity.

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Wei-Zhong Zhu

Department of Pharmacology
School of Medicine
Nantong University

19 Qi Xiu Road

Nantong 226001

People's Republic of China

Phone: + 86 5 13 85 05 17 28

Fax: + 86 5 13 85 05 18 58

Email: zhuwz@ntu.edu.cn