Planta Med 2009; 75(8): 870-872
DOI: 10.1055/s-0029-1185382
Biochemistry, Molecular Biology and Biotechnology
Letter
© Georg Thieme Verlag KG Stuttgart · New York

Molecular Authentication of the Traditional Medicinal Plant Fallopia multiflora

Chuan-Jin Zheng1 , Shu-Jin Zhao2 , Zhen-Hua Zhao1 , Jun Guo1
  • 1School of Biological Science and Engineering, South China University of Technology, Guangzhou, P. R. China
  • 2Department of Pharmacy, Guangzhou Liuhuaqiao Hospital, Guangzhou, P. R. China
Further Information

Publication History

received October 17, 2008 revised November 27, 2008

accepted January 19, 2009

Publication Date:
25 February 2009 (online)

Abstract

The root of Fallopia multiflora is one of the most widely used traditional Chinese medicines. However, it is often confused and substituted with the roots of F. multiflora var. ciliinervis, Pteroxygonum giraldii, Cynanchum auriculatum, and Stephania cepharantha. To establish a DNA polymorphism-based assay for the identification of F. multiflora, the nuclear ribosomal DNA (nrDNA) internal transcribed spacer (ITS) regions of six Fallopia species were sequenced and analyzed. Based on the diversity of ITS regions among the species the diagnostic primers PMITS28 and PMITS545, which amplified an expected 517-bp DNA fragment from F. multiflora DNA, were designed. No amplified product was observed when DNA from other species was used. This method can be used for the authentication of F. multiflora.

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Prof. Dr. Shu-Jin Zhao

Department of Pharmacy
Liuhuaqiao Hospital

Liuhua Road 111

Guangzhou 510010

Guangdong Province

People's Republic of China

Phone: + 86 20 36 65 34 77

Fax: + 86 20 36 65 34 77

Email: gzzsjzhs@163.com