RSS-Feed abonnieren
DOI: 10.1055/s-0029-1185319
© Georg Thieme Verlag KG Stuttgart · New York
Antinociceptive Effect and GC/MS Analysis of Rosmarinus officinalis L. Essential Oil from its Aerial Parts
Publikationsverlauf
received July 8, 2008
revised November 24, 2008
accepted December 7, 2008
Publikationsdatum:
30. Januar 2009 (online)
![](https://www.thieme-connect.de/media/plantamedica/200905/lookinside/thumbnails/10.1055-s-0029-1185319-1.jpg)
Abstract
The rationale of this investigation was to examine the antinociceptive properties of the essential oil obtained from Rosmarinus officinalis aerial parts, using a rat model of arthritic pain. The essential oil (100, 300 and 600 mg/kg, i. p.) produced a dose-dependent antinociceptive effect, manifested as a significant reduction in the dysfunction in the pain-induced functional impairment model in the rat (PIFIR model), mainly at high doses. Chemical constituents of the essential oil were further analyzed by gas chromatography-mass spectrometry (GC/MS). The major compounds in the essential oil were α-pinene (14.10 %), camphene (11.47 %), β-pinene (12.02 %), myrcene (3.31 %), α-phellandrene (7.87 %), eucalyptol (8.58 %), 2-bornanone (3.42 %), camphor (8.75 %), isoborneol (3.48 %), borneol (4.85 %) and borneol acetate (6.49 %). The antinociceptive effects of R. officinalis essential oil were tested in combination with 0.12 mg/kg WAY100635, s. c. (an antagonist of 5-HT1A receptors) or 1 mg/kg naloxone, i. p. (an antagonist of endogenous opioids receptors), demonstrating in both cases an inhibition of the antinociceptive response. This study suggests an involvement, at least in part, of the serotonergic system via 5-HT1A receptors and endogenous opioids in the antinociceptive effect of R. officinalis essential oil in the PIFIR model.
Key words
antinociception - endogenous opioids - PIFIR assay - 5‐HT1A receptors - Rosmarinus officinalis L. - Lamiaceae
References
- 1 Kim M J, Nam E S, Paik S I. The effects of aromatherapy on pain, depression, and life satisfaction of arthritis patients. Taehan Kanho Hakhoe Chi. 2005; 35 186-194
- 2 Gedney J J, Glover T L, Fillingim R B. Sensory and affective pain discrimination after inhalation of essential oils. Psychosom Med. 2004; 66 599-606
- 3 Santoyo S, Cavero S, Jaime L, Ibañez E, Señoráns F J, Reglero G. Chemical composition and antimicrobial activity of Rosmarinus officinalis L. essential oil obtained via supercritical fluid extraction. J Food Prot. 2005; 68 790-795
- 4 González-Trujano M E, Peña E I, Martínez A L, Guevara-Fefer P, Moreno J, Déciga-Campos M, López-Munñoz F J. Evaluation of the antinociceptive effect of Rosmarinus officinalis L. using three different experimental models in rodents. J Ethnopharmacol. 2007; 111 476-482
- 5 López-Muñoz F J, Salazar L A, Castañeda-Hernández G, Villarreal J E. A new model to assess analgesic activity: pain-induced functional impairment in the rat (PIFIR). Drug Dev Res. 1993; 28 169-175
- 6 Lukaczer D, Darland G, Tripp M, Liska D, Lerman R H, Schiltz B, Bland J S. A pilot trial evaluating Meta050, a proprietary combination of reduced iso-alpha acids, rosemary extract and oleanolic acid in patients with arthritis and fibromyalgia. Phytother Res. 2005; 19 864-869
- 7 Tung Y T, Chua M T, Wang S Y, Chang S T. Anti-inflammation activities of essential oil and its constituents from indigenous cinnamon (Cinnamomum osmophloeum) twigs. Bioresources Technol. 2008; 99 3908-3913
- 8 Chao L K, Hua K F, Hsu H Y, Cheng S S, Liu J Y, Chang S T. Study on the anti-inflammatory activity of essential oil from leaves of Cinnamomum osmophloeum. J Agric Food Chem. 2005; 53 7274-7278
- 9 Lee H J, Hyun E A, Yoon W J, Kim B H, Rhee M H, Kang H K, Cho J Y, Yoo E S. In vitro anti-inflammatory and anti-oxidative effects of Cinnamomum camphora extracts. J Ethnopharmacol. 2006; 103 208-216
- 10 Menezes I A, Marques M S, Santos T C, Dias K S, Silva A B, Mello I C, Lisboa A C, Alves P B, Cavalcanti S C, Marçal R M, Antoniolli A R. Antinociceptive effect and acute toxicity of the essential oil of Hyptis fruticosa in mice. Fitoterapia. 2007; 78 92-95
- 11 Aydin S, Demir T, Oztürk Y, Başer K H. Analgesic activity of Nepeta italica L. Phytother Res. 1999; 13 20-23
- 12 Liapi C, Anifandis G, Chinou I, Kourounakis A P, Theodosopoulos S, Galanopoulou P. Antinociceptive properties of 1,8-cineole and beta-pinene, from the essential oil of Eucalyptus camaldulensis leaves, in rodents. Planta Med. 2007; 73 1247-1254
- 13 Rao V S, Menezes A M, Viana G S. Effect of myrcene on nociception in mice. J Pharm Pharmacol. 1990; 42 877-878
- 14 Sousa O V, Silvério M S, Del-Vechio-Vieira G, Matheus F C, Yamamoto C H, Alves M S. Antinociceptive and anti-inflammatory effects of the essential oil from Eremanthus erythropappus leaves. J Pharm Pharmacol. 2008; 60 771-777
- 15 Santos F A, Rao V S. Antiinflammatory and antinociceptive effects of 1,8-cineole a terpenoid oxide present in many plant essential oils. Phytother Res. 2000; 14 240-244
- 16 Juergens U R, Engelen T, Racké K, Stöber M, Gillissen A, Vetter H. Inhibitory activity of 1,8-cineol (eucalyptol) on cytokine production in cultured human lymphocytes and monocytes. Pulm Pharmacol Ther. 2004; 17 281-287
- 17 Macpherson L J, Hwang S W, Miyamoto T, Dubin A E, Patapoutian A, Story G M. More than cool: promiscuous relationships of menthol and other sensory compounds. Mol Cell Neurosci. 2006; 32 335-343
- 18 Bang S, Kim K Y, Yoo S, Kim Y G, Hwang S W. Transient receptor potential A1 mediates acetaldehyde-evoked pain sensation. Eur J Neurosci. 2007; 26 2516-2523
- 19 Hall A C, Turcotte C M, Betts B A, Yeung W Y, Agyeman A S, Burk L A. Modulation of human GABAA and glycine receptor currents by menthol and related monoterpenoids. Eur J Pharmacol. 2004; 506 9-16
- 20 Zimmermann M. The guidelines on ethical standards for investigation of experimental pain in animals. Pain. 1983; 16 109-110
Dra. Ma. Eva González Trujano
Instituto Nacional de Psiquiatría “Ramón de la Fuente Muñiz”
Calz. México-Xochimilco
101 Col. Sn Lorenzo Huipulco
14370, México
D. F. México
Telefon: + 52 55 56 55 28 11
Fax: + 52 55 56 55 99 80
eMail: evag@imp.edu.mx