Acasiane A and B and Farnesirane A and B, Diterpene Derivatives From the Roots of Acacia farnesiana

Abstract

Four new diterpenes, acasiane A (1), acasiane B (2), farnesirane A (3), and farnesirane B (4), along with three known diterpenes (5 – 7), two triterpenes (8 and 9), and eight flavonoids (10 – 17) were isolated from the roots of Acacia farnesiana. The structures and relative configurations of these compounds were determined by various spectroscopic and x-ray analyses. All isolated compounds were evaluated for their in vitro cytotoxic activities against six human cancer cell lines (Hep G2, Hep 3B, MDA-MB-231, MCF-7, A549, and Ca9 – 22) with the MTT method. Betulinic acid (8) displayed moderate cytotoxicity (1.70 – 5.74 μg/mL) towards five human cancer cell lines and the flavonoids had slight effects. In addition, 8, diosmetin (13), and 3′,4′,5-trihydroxy-7-methoxyflavone (15) slightly inhibited superoxide anion generation or elastase release by human neutrophils, indicating moderate anti-inflammatory activities.

Key words

Mimosoideae - Acacia farnesiana - cassane diterpene - betulinic acid - cytotoxicity - anti-inflammatory

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