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Horm Metab Res 1970; 2(6): 349-351
DOI: 10.1055/s-0028-1095052
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© Georg Thieme Verlag KG Stuttgart · New York

Inhibition of Glyceraldehyde-3-Phosphate Dehydrogenase by Follicle-Stimulating Hormone Preparations In Vitro

T. T. T. Yen , M. C. Wacholtz [*] , M. M. Greenberg
  • The Biological Research Division, Eli Lilly and Company, Indianapolis, Indiana, U.S.A.
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Publication History

Publication Date:
08 January 2009 (online)

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Abstract

Rabbit muscle GAPDH (E.C.1.2.1.12) was inhibited by two preparations of FSH, one ovine and the other porcine. A third preparation, also of ovine origin, did not inhibit this enzyme. This suggested that the inhibition was not due to FSH but a contaminant. This inhibition of glyceraldehyde-3-phosphate dehydrogenase was specific. None of the other pituitary hormones had any effect on this enzyme and other NAD-linked and NADP-linked dehydrogenases were not inhibited by these FSH preparations.

Abbreviations used
ADH = alcohol dehydrogenase
EDTA= disodium ethylenedinitrilotetracetate
FSH = follicle-stimulating hormone
GDH = L-glutamic dehydrogenase
G6PDH = glucose-6-phosphate dehydrogenase
GAPDH = glyceraldehyde-3-phosphate dehydrogenase
LH = luteinizing hormone
α-MSH = α-melanocyte-stimulating hormone
6PGDH = 6-phosphogluconate dehydrogenase
STH = growth hormone
TSH = thyroid-stimulating hormone

Key words

Follicle-Stimulating Hormone - Glyceraldehyde 3-Phosphate Dehydrogenase - Growth Hormone - Pituitary Hormone

1 Present address: Department of Biological Chemistry, Harvard University Medical School, Boston, Massachusetts 0215