Human Bile Insulin Sephadex Fractions

Abstract

The significance of insulin Sephadex fractions in relation to insulin release was studied in two spontaneous bile fistulas in human beings by measuring the serum insulin and bile insulin release under the oral glucose and intravenous glucose infused at a constant rate. The results confirmed the presence of insulin in bile, and the changes during glucose tolerance do in fact indicate different behavior for oral glucose and glucose infusion. On the other hand, the findings clearly indicate the existence of two immunoreactive components in human bile. The two correspond to what Roth, Gorden and Pastan (1968) have described in human plasmas as big and little insulin. The results provided no support for the concept that a large fraction of big insulin is related to the high levels of immunoreactive bile insulin seen after oral glucose and intravenous administration.

Key words

Radioimmunoassay- IRI - Serum Insulin - Bile Insulin - Cholecystectomy - Gastrectomy - Fistula - Release - Big Insulin - Little Insulin - Bile Insulin Sephadex Fractions - Bile Clearance - Bile Excretion

1 Present address: Dept. Internal Medicine, School of Medicine, Yale University, 333 Cedar Street, New Haven, Conn. (U.S.A.)