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Horm Metab Res 1973; 5(4): 237-240
DOI: 10.1055/s-0028-1093957
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© Georg Thieme Verlag KG Stuttgart · New York

Immunological Comparison of Natural and Synthetic Secretins[*]

G.  Boden , V.  Dinoso , O. E. Owen
  • Department of Medicine and General Clinical Research Centre, Temple University Health Sciences Center, Philadelphia, Pa., U.S.A.
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Publication Date:
07 January 2009 (online)

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Abstract

Immunoreactivities of several natural and synthetic secretins were investigated using a newly developed radioimmunoassay. It was found that weight for weight immunologic activities of the purest natural (Mutt) and synthetic (Squibb) secretins were indistinguishable.

Compared to Squibb secretin, on a weight for weight basis, immunoreactivity of semipurified synthetic Schwarz/Mann secretin exceeded its bioactivity. This suggested contamination of Schwarz/Mann secretin with immunologically cross-reacting but biologically inactive peptide fragments.

Immunoreactivity of crude Sigma secretin was found to be about 104 times less than that of Mutt secretin.

The commercially available pure porcine secretin/cystein-HCl mixture (Jorpes) was found to be stable and to contain the equivalent of 21 µg of pure porcine secretin per ampule. After calibration against Squibb or Mutt secretin it can be used as reference standard in the secretin radioimmunoassay.

Key words

Radioimmunoassay - Immunoreactivity - Natural Secretin - Synthetic Secretin - Semipurified Secretin

1 This work was supported in part by grants from U.S. Public Health Service, Research Grant No. 5 MOI RR 349, National Institutes of Health, General Clinical Research Centers Branch.

1 This work was supported in part by grants from U.S. Public Health Service, Research Grant No. 5 MOI RR 349, National Institutes of Health, General Clinical Research Centers Branch.