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Horm Metab Res 1974; 6(1): 12-16
DOI: 10.1055/s-0028-1093895
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© Georg Thieme Verlag KG Stuttgart · New York

A Simple Free Fat Cell Bioassay for Insulin

A. J. Moody1 , M. A. Stan1 , M.  Stan1 [*] , J.  Gliemann2
  • 1Novo Research Institute, Novo Allé, Bagsvaerd, Denmark
  • 2Institute of Medical Physiology C, University of Copenhagen, Copenhagen, Denmark
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Publication History

Publication Date:
07 January 2009 (online)

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Abstract

A simple bioassay is described for insulin-like activity (ILA) which is based on the incorporation of (3H)glucose into the lipids of isolated rat fat cells. Total lipids were extracted by adding toluene-based scintillation fluid directly to the incubation flasks, and the radioactivity in the lipids measured by liquid scintillation counting. Insulin dose-response curves with (2-3H)-, (3-3H) and (6-3H)glucose were compared and those obtained with (2-3H)glucose and (3-3H)glucose were found suitable for routine use.

Key words

Insulin - Free Fat Cells - Bioassay - (3H)Glucose - (SH)Lipid Extraction - (3H)Lipid Counting

1 Present address: Rheumaklinik Landesbad, Burtscheider Markt 24, D-51 Aachen.