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DOI: 10.1055/s-0028-1088334
© Georg Thieme Verlag KG Stuttgart · New York
Identification of Crude Drugs from Chinese Medicinal Plants of the Genus Bupleurum Using Ribosomal DNA ITS Sequences
Publication History
Received: September 20, 2007
Revised: August 25, 2008
Accepted: September 23, 2008
Publication Date:
18 November 2008 (online)
Abstract
To determine the variation of rDNA ITS sequences in medicinal plants of the genus Bupleurum and identify corresponding DNA molecular markers associated with medicinally important members, ITS regions were amplified, sequenced, and analyzed by DNAssist version 2.2. We found that the homologous alignment of ITS sequences between members of the genus Bupleurum and associated out-groups was lower than 75 %, while within-group alignment was greater than 87 %. The conclusion can be drawn that ITS sequences can be used as reliable molecular markers for the identification of Radix Bupleuri.
Key words
ITS sequence - Chaihu - Radix Bupleuri - Bupleurum - Umbelliferae (Apiaceae) - identification
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Prof. Shengli Pan
School of Pharmacy
Fudan University
138 Yixueyuan Road
Shanghai 200032
People’s Republic of China
Phone: +86-21-5423-7454
Email: panshengli@online.sh.cn