Planta Med 2008; 74 - PC71
DOI: 10.1055/s-0028-1084589

The rapid analysis and identification of prenylflavonoids in Sophora flavescens roots by on flow LC-NMR/MS

MC Kim 1, 2, SW Kang 1, SJ Kim 1, 3, BH Um 1
  • 1Natural Product Research Center, Korea Institute of Science and Technology Gangneung Institute, Gangneung, Gangwon-do, 210–340, Korea
  • 2Department of Biology, Kangnung National University, Gangneung 210–702, Korea
  • 3Department of Chemistry, Kangnung National University, Gangneung 210–702, Korea

The hyphenated techniques of LC-NMR/MS are increasingly used for phytochemical analysis. These techniques combine the separation power of HPLC with the structural information provided by NMR and MS. The major advantages over the traditional off-line techniques are rapidity and efficiency. The application of LC-NMR/MS and HPLC on-line ABTS antioxidant screening system for the direct identification of prenylflavonoids in Sophora flavescens has been studied. The antioxidant compounds of S. flavescens were revealed by HPLC on-line ABTS antioxidant screening system which composed of ABTS post-column derivatization. Six prenylflavonoids were identified as kushenol U (1), kurarinone (2), sophoraflavanone G (3), leachianone A (4), kuraridin (5), and kushenol A (6) using on flow LC-NMR/MS.

Acknowledgements: Grant No. RTI05–01–02 from the Regional Technology Innovation Program of the Ministry of Knowledge Economy (MKE)