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Planta Med 2024; 90(11): 885-895
DOI: 10.1055/a-2341-6175
Biological and Pharmacological Activity
Original Papers

5(S)-5-Carboxystrictosidine from the Root of Mappianthus iodoides Ameliorates H2O2-induced Apoptosis in H9c2 Cardiomyocytes via PI3K/AKT and ERK Pathways

Ying Han‡
1   Department of Physiology, College of Chinese Medicine and Life Science, Jiangxi University of Chinese Medicine, Nanchang City, Jiangxi Province, China
5   Key Laboratory of Psychology of TCM and Brain Science, Jiangxi Administration of traditional Chinese Medicine, Jiangxi University of Chinese Medicine, Nanchang City, Jiangxi Province, China
,
Junli Xi‡
1   Department of Physiology, College of Chinese Medicine and Life Science, Jiangxi University of Chinese Medicine, Nanchang City, Jiangxi Province, China
,
Puzhao Zhang
2   Key Laboratory of Innovation Drug and Efficient Energy-saving Pharmaceutical Equipment, Jiangxi University of Chinese Medicine, Nanchang City, Jiangxi Province, China
,
Ming Gong
1   Department of Physiology, College of Chinese Medicine and Life Science, Jiangxi University of Chinese Medicine, Nanchang City, Jiangxi Province, China
,
Tao Luo
3   Blood Purification Center of the First Affiliated Hospital of Nanchang University, Nanchang City, Jiangxi Province, China
,
Feng Shao
2   Key Laboratory of Innovation Drug and Efficient Energy-saving Pharmaceutical Equipment, Jiangxi University of Chinese Medicine, Nanchang City, Jiangxi Province, China
,
Yongxin Li
1   Department of Physiology, College of Chinese Medicine and Life Science, Jiangxi University of Chinese Medicine, Nanchang City, Jiangxi Province, China
,
Lingyun Zhong
4   College of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang City, Jiangxi Province, China
,
Hexiu Quan
1   Department of Physiology, College of Chinese Medicine and Life Science, Jiangxi University of Chinese Medicine, Nanchang City, Jiangxi Province, China
› InstitutsangabenGefördert durch: Top Discipline of Jiangxi Province，Discipline of Chinese and Western Integrative Medicine, Jiangxi University of Chinese Medicine zxyylxk20220103
Gefördert durch: The Graduate Student Innovation special Fund Project of Jiangxi Province YC2021-S485
Gefördert durch: Inherited and Innovative Group of Processing Technique of Traditional Chinese Medicine CXTD22003
Gefördert durch: Science and Technology Project of Jiangxi Provincial Health Commission 202211410
Gefördert durch: The Science and Technology Research Project of Jiangxi Provincial Department of Education, China GJJ2200958
Gefördert durch: The Science and Technology Research Project of Jiangxi Provincial Department of Education, China GJJ211204
Gefördert durch: Innovation and Entrepreneurship training program for college students in Jiangxi Province X202310412181
Gefördert durch: Science and technology project of Jiangxi Provincial Administration of Traditional Chinese Medicine 2023B1182
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Abstract

5(S)-5-carboxystrictosidine (5-CS) is a compound found in the root of Mappianthus iodoides, a traditional Chinese medicine used for the treatment of coronary artery disease. The aim of the present study was to investigate the protective effect of 5-CS against oxidative stress-induced apoptosis in H9c2 cardiomyocytes and the underlying mechanisms. 5-CS pretreatment significantly protected against H2O2-induced cell death, LDH leakage, and malondialdehyde (MDA) production, which are indicators for oxidative stress injury. 5-CS also enhanced the activity of SOD and CAT. In addition, 5-CS pretreatment significantly inhibited H2O2-induced apoptosis, as determined by flow cytometer, suppressed the activity of caspase-3 and caspase-9, and attenuated the activation of cleaved caspase-3 and caspase-9. 5-CS also increased Akt and ERK activation altered by H2O2 using Western blot analysis. The PI3K-specific inhibitor LY294002 abolished 5-CS-induced Akt activation. The ERK-specific inhibitor PD98059 abolished 5-CS-induced ERK activation. Both LY294002 and PD98059 attenuated the protective effect of 5-CS on H9c2 cardiomyocytes against H2O2-induced apoptosis and cell death. Taken together, these results demonstrate that 5-CS prevents H2O2-induced oxidative stress injury in H9c2 cells by enhancing the activity of the endogenous antioxidant enzymes, inhibiting apoptosis, and modulating PI3K/Akt and ERK signaling pathways.

Keywords

5(S)-5-carboxystrictosidine - Mappianthus iodoides - Icacinaceae - myocardial apoptosis - reactive oxygen species - PI3K/Akt - ERK

Supporting Information

    The extraction method of 5-CS from Mappianthus iodoides and the effects used in the Western blotting assay are described in the supplemental material.

  • Ergänzendes Material


Publikationsverlauf

Eingereicht: 14. November 2023

Angenommen nach Revision: 10. Juni 2024

Accepted Manuscript online:
10. Juni 2024

Artikel online veröffentlicht:
19. Juli 2024

© 2024. Thieme. All rights reserved.

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