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Semin Liver Dis
DOI: 10.1055/a-2299-7880
Review Article

Emerging Roles of Spatial Transcriptomics in Liver Research

Naoto Fujiwara‡   *
1   Department of Gastroenterology and Hepatology, Graduate School of Medicine, Mie University, Mie, Japan
,
Genki Kimura‡   *
1   Department of Gastroenterology and Hepatology, Graduate School of Medicine, Mie University, Mie, Japan
,
Hayato Nakagawa
1   Department of Gastroenterology and Hepatology, Graduate School of Medicine, Mie University, Mie, Japan
› Author Affiliations Funding This research was funded by the Japan Society for the Promotion of Science KAKENHI Grant Number 21H02892, AMED Grant Number JP23fk0210130, JP23fk0210090, JP23fk0210115, the Princess Takamatsu Cancer Research Fund, Daiichi Sankyo Foundation of Life Science, and Takeda Science Foundation to H.N.
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Abstract

Spatial transcriptomics, leveraging sequencing- and imaging-based techniques, has emerged as a groundbreaking technology for mapping gene expression within the complex architectures of tissues. This approach provides an in-depth understanding of cellular and molecular dynamics across various states of healthy and diseased livers. Through the integration of sophisticated bioinformatics strategies, it enables detailed exploration of cellular heterogeneity, transitions in cell states, and intricate cell–cell interactions with remarkable precision. In liver research, spatial transcriptomics has been particularly revelatory, identifying distinct zonated functions of hepatocytes that are crucial for understanding the metabolic and detoxification processes of the liver. Moreover, this technology has unveiled new insights into the pathogenesis of liver diseases, such as the role of lipid-associated macrophages in steatosis and endothelial cell signals in liver regeneration and repair. In the domain of liver cancer, spatial transcriptomics has proven instrumental in delineating intratumor heterogeneity, identifying supportive microenvironmental niches and revealing the complex interplay between tumor cells and the immune system as well as susceptibility to immune checkpoint inhibitors. In conclusion, spatial transcriptomics represents a significant advance in hepatology, promising to enhance our understanding and treatment of liver diseases.

Keywords

spatial transcriptomics - hepatic zonation - cell deconvolution - liver cancer - immune checkpoint inhibitor

* Equally contributed.




Publication History

Accepted Manuscript online:
04 April 2024

Article published online:
30 April 2024

© 2024. Thieme. All rights reserved.

Thieme Medical Publishers, Inc.
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