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Planta Med 2023; 89(11): 1087-1096
DOI: 10.1055/a-2072-2351
Natural Product Chemistry and Analytical Studies
Original Papers

Metabolic Fingerprinting of Different Sideritis Taxa Infusions and Their Neurogenic Activity

Ekaterina-Michaela Tomou
1   Section of Pharmacognosy & Chemistry of Natural Products, Department of Pharmacy, School of Health Sciences, National and Kapodistrian University of Athens, Panepistimiopolis, Zografou, Athens, Greece
2   Weihenstephan-Triesdorf University of Applied Sciences, Organic-Analytical Chemistry, Straubing, Germany
,
Lara Bieler
3   Institute of Experimental Neuroregeneration, Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University Salzburg, Salzburg, Austria
4   Austrian Cluster for Tissue Regeneration, Vienna, Austria
,
Tobias Spöttl
5   TUM Campus Straubing for Biotechnology and Sustainability, Technical University of Munich, Straubing, Germany
,
Sebastien Couillard-Despres
3   Institute of Experimental Neuroregeneration, Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University Salzburg, Salzburg, Austria
4   Austrian Cluster for Tissue Regeneration, Vienna, Austria
,
Helen Skaltsa 
1   Section of Pharmacognosy & Chemistry of Natural Products, Department of Pharmacy, School of Health Sciences, National and Kapodistrian University of Athens, Panepistimiopolis, Zografou, Athens, Greece
,
Corinna Urmann 
2   Weihenstephan-Triesdorf University of Applied Sciences, Organic-Analytical Chemistry, Straubing, Germany
5   TUM Campus Straubing for Biotechnology and Sustainability, Technical University of Munich, Straubing, Germany
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Abstract

Over the last years, Sideritis extracts were shown to improve memory. However, their potential to promote the generation of new neurons, starting with the neuronal differentiation of neural stem cells, remains unexplored. Therefore, the present study aimed to evaluate the neurogenic effects of different Sideritis infusions in neural stem and precursor cells and their impact on cell viability. Moreover, the metabolic fingerprints were recorded using LC-DAD, LC-HRESIMS, and GC-MS. The neurogenic potential of infusions of the eight Sideritis taxa tested was as potent as the classical neuronal inducer combination of retinoic acid and valproic acid. Further cytotoxicity assays revealed that the IC50 values of the extracts were between 163 and 322 µg/mL. Hierarchical cluster analyses of the metabolic fingerprints unveiled that the two Sideritis taxa with the lowest IC50 values were the most divergent in the analytical techniques used. As the analysis focused on polyphenols, it is reasonable to assume that these compounds are responsible for the effect on the cell viability of SH-SY5Y neuroblastoma cells. This study is the first report on the neurogenic potential of Sideritis taxa and might support the use of Sideritis herbal preparations in the context of neurodegenerative diseases.

Key words

Sideritis - Lamiaceae - neurogenesis - doublecortin - infusions - metabolic fingerprint


Publikationsverlauf

Eingereicht: 29. Dezember 2022

Angenommen nach Revision: 11. April 2023

Accepted Manuscript online:
12. April 2023

Artikel online veröffentlicht:
23. Mai 2023

© 2023. Thieme. All rights reserved.

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

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