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Planta Med 2023; 89(04): 408-415
DOI: 10.1055/a-1923-4399
Biological and Pharmacological Activity
Original Papers

Resveratrol Protects BEAS-2B Cells against Erastin-Induced Ferroptosis through the Nrf2/Keap1 Pathway

Wenhan Huang
1   The Second Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China
,
Liuda Yu
1   The Second Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China
,
Wanru Cai
1   The Second Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China
,
Chunfang Ma
2   Laboratory Medicine Center, Department of Clinical Laboratory, Zhejiang Provincial Peopleʼs Hospital, Affiliated Peopleʼs Hospital, Hangzhou Medical College, Hangzhou, Zhejiang, China
› Author AffiliationsSupported by: Zhejiang Provincial Medicine and Health Research Foundation 2020374897
Supported by: National Natural Science Foundation of China 81774220
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Abstract

Ferroptosis is a newly discovered type of cell death that is different from other types of cell death morphologically and biologically. It is considered to play an important role in many pulmonary diseases. Currently, the regulatory roles of antioxidation in lung epithelial ferroptosis have not been fully explored. In this study, we show that resveratrol protected erastin-induced ferroptosis in BEAS-2B cells. Erastin led to increased reactive oxygen species production and iron deposition in BEAS-2B cells, which could be rescued by resveratrol. Furthermore, we observed that resveratrol led to modulating ferroptosis-associated gene glutathione peroxidase 4 expression and regulating glutathione in BEAS-2B cells. Resveratrol exerted an antioxidant property in erastin-induced ferroptosis of BEAS-2B cells by activating the nuclear factor-erythroid 2-related factor 2/Kelch-like ECH-associated protein signaling pathway. Finally, these findings demonstrate that resveratrol protects BEAS-2B from erastin-induced ferroptosis.

Key words

Liliaceae - ferroptosis - resveratrol - antioxidation - reactive oxygen species - nuclear factor-erythroid 2-related factor 2 - Kelch-like ECH-associated protein 1


Publication History

Received: 18 April 2022

Accepted after revision: 03 August 2022

Article published online:
27 September 2022

© 2022. Thieme. All rights reserved.

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

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