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J Reconstr Microsurg 2023; 39(04): 311-319
DOI: 10.1055/a-1896-5471
Original Article

The Effect of Vascular Endothelial Growth Factor C and Adipose-Derived Stem Cells on Lymphatic Regeneration in a Rat Vascularized Lymph Node Transfer Model

Filip Jonas
1   Department of Plastic Surgery, University Hospital Bulovka, First Faculty of Medicine, Charles University, Prague, Czech Republic
2   Department of Plastic Surgery, Third Faculty of Medicine, Teaching Hospital Královské Vinohrady, Charles University, Prague, Czech Republic
,
Peter Kesa
3   Center for Advanced Preclinical Imaging (CAPI), First Faculty of Medicine, Charles University, Prague, Czech Republic
,
Petr Paral
3   Center for Advanced Preclinical Imaging (CAPI), First Faculty of Medicine, Charles University, Prague, Czech Republic
,
Jan Pankrac
3   Center for Advanced Preclinical Imaging (CAPI), First Faculty of Medicine, Charles University, Prague, Czech Republic
,
Marie Hubalek Kalbacova
4   Institute of Pathological Physiology, First Faculty of Medicine, Charles University, Prague, Czech Republic
,
Jakub Miletin
2   Department of Plastic Surgery, Third Faculty of Medicine, Teaching Hospital Královské Vinohrady, Charles University, Prague, Czech Republic
,
Andrej Sukop
2   Department of Plastic Surgery, Third Faculty of Medicine, Teaching Hospital Královské Vinohrady, Charles University, Prague, Czech Republic
,
Martin Molitor
1   Department of Plastic Surgery, University Hospital Bulovka, First Faculty of Medicine, Charles University, Prague, Czech Republic
,
Krystof Stanek 
1   Department of Plastic Surgery, University Hospital Bulovka, First Faculty of Medicine, Charles University, Prague, Czech Republic
,
Ludek Sefc
3   Center for Advanced Preclinical Imaging (CAPI), First Faculty of Medicine, Charles University, Prague, Czech Republic
,
Ondrej Mestak
1   Department of Plastic Surgery, University Hospital Bulovka, First Faculty of Medicine, Charles University, Prague, Czech Republic
› Institutsangaben Funding This work was performed under support of grant AZV 17-29084A by the Ministry of Health of the Czech Republic. Marie Hubalek Kalbacova is grateful to Charles University institutional support by grant PROGRES Q26/LF1. The imaging part of the work was supported by Large Infrastructure Support (National Infrastructure for Biological and Medical Imaging—Czech-BioImaging project No. LM 2018129) funded by the Ministry of Education, Youth, and Sport of the Czech Republic. The technical infrastructure was supported by European Regional Development Fund No. CZ.02.01./0.0./0.0./16_013/0001775.
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Abstract

Background Lymphedema is a chronic condition characterized by progressive edema with complicated treatment. Recently, new treatment strategies inducing lymphangiogenesis were proposed. The aim of our study was to examine the effect of vascular endothelial growth factor C (VEGF-C) and adipose-derived stem cells (ADSCs) on lymphatic regeneration and drainage re-establishment in vascularized lymph node transfer (VLNT) model using a pedicled vascularized lymph node (VLN) groin flap.

Methods Female Lewis rats with groin VLN flaps were utilized as a lymphedema model. Group A served as the control. Group B received VEGF-C. Group C received both VEGF-C and ADSCs. Group D received ADSCs only. Lymphatic drainage re-establishment was evaluated by ultrasound–photoacoustic imaging (US-PAI) after indocyanine green (ICG) injection.

Results The fastest regeneration of elevated flaps was observed in Groups B and C in all monitored periods. After the first month, ICG positivity was detected in 14.3% of animals in Group A, 71.43% of animals in Group B (odds ratio [OR] = 15; p = 0.048), and 83.33% in Group C (OR = 30; p = 0.027). On the contrary, the difference between control group and Group D (16.67%; p = 0.905) was statistically insignificant. Administration of VEGF-C, ADSC + VEGF-C, and ADSC led to full flap regeneration after 6 months. The control group had the lowest percentage of ICG positivity at all monitored time points.

Conclusion We found that the fastest regeneration occurred with the combination of the VLN flap and VEGF-C. The addition of ADSC had an insignificant effect in our study. Furthermore, we proved the feasibility of PAI as an assessment tool of the lymphatic drainage recovery in a VLNT model.

Keywords

VEGF - ADSC - vascularized lymph node transfer - VLNT - lymphatic regeneration

Ethics Approval and Consent to Participate

This study was performed in strict accordance with the Institutional Animal Care and Use Committee (MSMT-9993/2017-4). All surgeries were performed under anesthesia, and all efforts were made to minimize suffering.


Availability of Data and Materials

All data generated or analyzed during this study are included in this published article.


Authors' Contribution

F.J. contributed to conception design of the work; animal experiments, acquisition, analysis, interpretation of data; and drafted the work. P.K., P.P., and J.P. helped in acquisition, analysis, and interpretation of imaging data. M.H.K. helped in acquisition, analysis, and interpretation of data in stem cells experiments and drafted the work or substantively revised it. J.M. performed animal experiments. A.S. and M.M. contributed to conception design of the work. K.S. drafted the work. L.S. contributed to conception design of the work and revision. O.M. contributed to conception design of the work; helped in analysis and interpretation of data; and drafted the work and substantively revised it. All authors read and approved the final manuscript.




Publikationsverlauf

Eingereicht: 01. Februar 2022

Angenommen: 29. Juni 2022

Accepted Manuscript online:
11. Juli 2022

Artikel online veröffentlicht:
22. November 2022

© 2022. Thieme. All rights reserved.

Thieme Medical Publishers, Inc.
333 Seventh Avenue, 18th Floor, New York, NY 10001, USA

 

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