Rhodol-Based Fluorescent Probes Used for Fast Response toward ClO^– and Delayed Determination of H₂O₂ in Living Cells

Abstract

Reactive oxygen species (ROS), a class of reactive oxidants, play critical roles in signal transduction, cell metabolism, immune defense, and other physiological processes. Abnormally excessive levels of ROS can cause diseases and thus, investigations into the relevant biology and medicine are significant. The behavior of ROS in inflammation has been rarely elucidated. In this work, two ROS fluorescent probes, FS-ROS1 and FS-ROS2 have been designed and synthesized. FS-ROS1 responds rapidly (~1 min) to ClO^– and gradually (~30 min) to H₂O₂ with an increase in fluorescence at ~656 nm and 640 nm of more than 100-fold in vitro. At a concentration of 10 μM, FS-ROS1 labels the L929 cell and Raw264.7 cell wells in 30 min with excellent biocompatibility and without washing. After labelling, FS-ROS1 exhibited a rational fluorescence increase upon the addition of 1, 10, 100, and 200 μM of H₂O₂. Based on these results, inflammatory cells, stimulated with 800 nM dexamethasone and polyIC, showed a higher increase in fluorescence than the control cells. These results suggest that H₂O₂ and ClO^– might be important signaling molecules during inflammations.

Key words

ROS - rhodol - fluorescence - living cell imaging - inflammation

Publication History

Received: 25 January 2021

Accepted after revision: 02 March 2022

Accepted Manuscript online:
02 March 2022

Article published online:
19 April 2022

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