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Planta Med 2021; 87(12/13): 1089-1100
DOI: 10.1055/a-1554-2733
Natural Product Chemistry and Analytical Studies
Original Papers

Cytotoxic, Antioxidant, and Antidiabetic Activities versus UPLC-ESI-QTOF-MS Chemical-Profile Analysis of Ipomoea aquatica Fractions[ # ]

Mahmoud Hefny Gad
1   Analytical Chemistry, Applied Chemometrics and Molecular Modelling, Vrije Universiteit Brussel (VUB), Brussels, Belgium
2   Medicinal and Aromatic Plants Research Department, Horticulture Institute, Agricultural Research Center, Dokki, Giza, Egypt
,
Kristiaan Demeyer
3   Experimental In Vitro Toxicology and Dermato-Cosmetology, Vrije Universiteit Brussel (VUB), Brussels, Belgium
,
Yvan Vander Heyden
1   Analytical Chemistry, Applied Chemometrics and Molecular Modelling, Vrije Universiteit Brussel (VUB), Brussels, Belgium
,
Debby Mangelings
1   Analytical Chemistry, Applied Chemometrics and Molecular Modelling, Vrije Universiteit Brussel (VUB), Brussels, Belgium
› InstitutsangabenGefördert durch: Fonds Wetenschappelijk Onderzoek G033816N
Gefördert durch: Mundus MEDASTAR (Mediterranean Area for Science, Technology and Research) Mundus MEDASTAR/Grant agreement number (2011-4051/002-001-EMA2)
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Abstract

Ipomoea aquatica is a common green leafy vegetable that has numerous uses in traditional medicine. This study focused on the determination of the cytotoxic, antiradical, and antidiabetic properties of various fractions of the I. aquatica methanolic extract, as well as on the tentative identification of some bioactive compounds in the same fractions. The cytotoxicity was determined by the brine shrimp lethal test. The antioxidant activities of the I. aquatica fractions were investigated through 3 assays. The antidiabetic activity (in vitro) was measured by α-glucosidase and α-amylase inhibition assays. Phytochemical qualitative analyses demonstrated the presence of alkaloids, terpenoids, phenols, and flavonoids in the ethyl acetate-methanol and methanol fractions. The total phenolic and total flavonoid contents were found to be highest in the ethyl acetate-MeOH fractions. The evaluation of the cytotoxicity showed that the hexane-dichloromethane fraction is the most toxic, while the others are moderately toxic. The antioxidant activity assays showed that the ethyl acetate-MeOH fractions are the most potent, while the α-glucosidase and α-amylase assays revealed that the hexane-dichloromethane fraction might contain a potent antidiabetic agent. Some bioactive substances in the MeOH fractions, such as salicylic acid glucoside, 1-O-sinapoyl-β-D-glucose derivative, and dihydroferulic acid derivative, were tentatively identified. To the best of our knowledge, this is the first report to detect and identify these compounds in this species. Based on the results of this study, it may be concluded that I. aquatica is a potent antioxidant agent and could be a good candidate as a natural antioxidant in food and therapeutics.

Key words

Ipomoea aquatica - Convolvulaceae - chemical-profile analysis - cytotoxic activity - antioxidant activity - antidiabetic activity

# Dedicated to Professor Arnold Vlietinck on the occasion of his 80th birthday.




Publikationsverlauf

Eingereicht: 31. Januar 2021

Angenommen nach Revision: 09. Juli 2021

Artikel online veröffentlicht:
06. August 2021

© 2021. Thieme. All rights reserved.

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  • References

  • 1 Maiti R, Jana D, Das UK, Ghosh D. Antidiabetic effect of aqueous extract of seed of Tamarindus indica in streptozotocin-induced diabetic rats. J Ethnopharmacol 2004; 92: 85-91
    CrossrefPubMedGoogle Scholar
  • 2 Cordell GA. Phytochemistry and traditional medicine–the revolution continues. Phytochem Lett 2014; 10: xxviii-xl
    CrossrefPubMedGoogle Scholar
  • 3 Gazioglu I, Zengin OS, Tartaglia A, Locatelli M, Furton KG, Kabir A. Determination of polycyclic aromatic hydrocarbons in nutritional supplements by fabric phase sorptive extraction (FPSE) with high-performance liquid chromatography (HPLC) with fluorescence detection. Anal Lett 2021; 54: 1683-1696
    CrossrefPubMedGoogle Scholar
  • 4 Melucci D, Locatelli M, De Laurentiis F, Zengin G, Locatelli C. Herbal medicines: Application of a sequential voltammetric procedure to the determination of mercury, copper, lead, cadmium and zinc at trace level. Lett Drug Des Discov 2018; 15: 270-280
    CrossrefPubMedGoogle Scholar
  • 5 Jayaweera DMA. Medicinal Plants (Indigenous and Exotic) used in Ceylon. Colombo, Sri Lanka: National Science Council; 1980: 99-100
    Google Scholar
  • 6 Iwu MM. Handbook of African Medicinal Plants. Boca Raton, FL: CRC Press; 1993
    Google Scholar
  • 7 Malalavidhane S, Wickramasinghe SMDN, Jansz ER. An aqueous extract of the green leafy vegetable Ipomoea aquatica is as effective as the oral hypoglycaemic drug tolbutamide in reducing the blood sugar levels of Wistar rats. Phytother Res 2001; 15: 635-637
    CrossrefPubMedGoogle Scholar
  • 8 Prasad KN, Divakar S, Shivamurthy GR, Aradhya SM. Isolation of a free radical-scavenging antioxidant from water spinach (Ipomoea aquatica Forsk). J Sci Food Agric 2005; 85: 1461-1468
    CrossrefPubMedGoogle Scholar
  • 9 Kapoor D, Singh S, Kumar V, Romero R, Prasad R, Singh J. Antioxidant enzymes regulation in plants in reference to reactive oxygen species (ROS) and reactive nitrogen species (RNS). Plant Gene 2019; 19: 100182
    CrossrefPubMedGoogle Scholar
  • 10 Apak R, Gorinstein S, Böhm V, Schaich KM, Özyürek M, Güçlü K. Methods of measurement and evaluation of natural antioxidant capacity/activity (IUPAC Technical Report). Pure Appl Chem 2013; 85: 957-998
    CrossrefPubMedGoogle Scholar
  • 11 Etxeberria U, de la Garza AL, Campión J, Martínez JA, Milagro FI. Antidiabetic effects of natural plant extracts via inhibition of carbohydrate hydrolysis enzymes with emphasis on pancreatic alpha amylase. Expert Opin Ther Targets 2012; 16: 269-297
    CrossrefPubMedGoogle Scholar
  • 12 Datta S, Sinha BK, Bhattacharjee S, Seal T. Nutritional composition, mineral content, antioxidant activity and quantitative estimation of water soluble vitamins and phenolics by RP-HPLC in some lesser used wild edible plants. Heliyon 2019; 5: e01431
    CrossrefPubMedGoogle Scholar
  • 13 Musila MF, Dossaji SF, Nguta JM, Lukhoba CW, Munyao JM. In vivo antimalarial activity, toxicity and phytochemical screening of selected antimalarial plants. J Ethnopharmacol 2013; 146: 557-561
    CrossrefPubMedGoogle Scholar
  • 14 Octaviani CD, Lusiana M, Zuhrotun A, Diantini A, Subarnas A, Abdulah R. Anticancer properties of daily-consumed vegetables Amaranthus spinosus, Ipomoea aquatica, Apium graveolens, and Manihot utilisima to LNCaP prostate cancer cell lines. J Nat Pharmaceut 2013; 4: 67-70
    CrossrefPubMedGoogle Scholar
  • 15 Ullah MO, Haque M, Urmi KF, Zulfiker AH, Anita ES, Begum M, Hamid K. Anti-bacterial activity and brine shrimp lethality bioassay of methanolic extracts of fourteen different edible vegetables from Bangladesh. Asian Pac J Trop Biomed 2013; 3: 1-7
    CrossrefPubMedGoogle Scholar
  • 16 Doka GI, Tigni SE, Yagi S. Nutritional composition and antioxidant properties of Ipomoea aquatica (Forsek) leaves. J Forest Prod Ind 2014; 3: 204-210
    PubMedGoogle Scholar
  • 17 Zengin G, Locatelli M, Stefanucci A, Macedonio G, Novellino E, Mirzaie S, Dvorácskó S, Carradori S, Brunetti L, Orlando G. Chemical characterization, antioxidant properties, anti-inflammatory activity, and enzyme inhibition of Ipomoea batatas L. leaf extracts. Int J Food Prop 2017; 20: 1907-1919
    PubMedGoogle Scholar
  • 18 Zanfini A, Corbini G, La Rosa C, Dreassi E. Antioxidant activity of tomato lipophilic extracts and interactions between carotenoids and α-tocopherol in synthetic mixtures. LWT 2010; 43: 67-72
    CrossrefPubMedGoogle Scholar
  • 19 Huang D, Ou B, Hampsch-Woodill M, Flanagan JA, Deemer EK. Development and validation of oxygen radical absorbance capacity assay for lipophilic antioxidants using randomly methylated β-cyclodextrin as the solubility enhancer. J Agric Food Chem 2002; 50: 1815-1821
    CrossrefPubMedGoogle Scholar
  • 20 Prior RL, Hoang H, Gu L, Wu X, Bacchiocca M, Howard L, Hampsch-Woodill M, Huang D, Ou B, Jacob R. Assays for Hydrophilic and lipophilic antioxidant capacity (oxygen radical absorbance capacity (ORACFL)) of plasma and other biological and food samples. J Agric Food Chem 2003; 51: 3273-3279
    CrossrefPubMedGoogle Scholar
  • 21 Huang D, Ou B, Prior RL. The chemistry behind antioxidant capacity assays. J Agric Food Chemistry 2005; 53: 1841-1856
    CrossrefPubMedGoogle Scholar
  • 22 Deshpande MC, Venkateswarlu V, Babu RK, Trivedi RK. Design and evaluation of oral bioadhesive controlled release formulations of miglitol, intended for prolonged inhibition of intestinal α-glucosidases and enhancement of plasma glucagon like peptide-1 levels. Int J Pharm 2009; 380: 16-24
    CrossrefPubMedGoogle Scholar
  • 23 Dangi K, Mishra S. Antihyperglycemic, antioxidant and hypolipidemic effect of Capparis aphylla stem extract in streptozotocin induced diabetic rats. Biol Med 2010; 2: 35-44
    PubMedGoogle Scholar
  • 24 Lawal U, Leong SW, Shaari K, Ismail IS, Khatib A, Abas F. α-Glucosidase Inhibitory and antioxidant activities of different Ipomoea aquatica cultivars and LC-MS/MS Profiling of the active cultivar. J Food Biochem 2017; 41: e12303-12311
    CrossrefPubMedGoogle Scholar
  • 25 Gowri PM, Tiwari AK, Ali AZ, Rao JM. Inhibition of α-glucosidase and amylase by bartogenic acid isolated from Barringtonia racemosa Roxb. seeds. Phytother Res 2007; 21: 796-799
    CrossrefPubMedGoogle Scholar
  • 26 Ranilla LG, Kwon YI, Apostolidis E, Shetty K. Phenolic compounds, antioxidant activity and in vitro inhibitory potential against key enzymes relevant for hyperglycemia and hypertension of commonly used medicinal plants, herbs and spices in Latin America. Bioresour Technol 2010; 101: 4676-4689
    CrossrefPubMedGoogle Scholar
  • 27 Kwon YI, Jang HD, Shetty K. Evaluation of Rhodiold crenulata and Rhodiold rosea for management of Type II diabetes and hypertension. Asia Pacific J Clin Nutr 2006; 15: 425-432
    PubMedGoogle Scholar
  • 28 Rubilar M, Jara C, Poo Y, Acevedo F, Gutierrez C, Sineiro J, Shene C. Extracts of Maqui (Aristotelia chilensis) and Murta (Ugni molinae Turcz.): Sources of antioxidant compounds and α-glucosidase/α-amylase inhibitors. J Agric Food Chem 2011; 59: 1630-1637
    CrossrefPubMedGoogle Scholar
  • 29 Wang Y, Yang Z, Wei X. Sugar compositions, α-glucosidase inhibitory and amylase inhibitory activities of polysaccharides from leaves and flowers of Camellia sinensis obtained by different extraction methods. Int J Biol Macromol 2010; 47: 534-539
    CrossrefPubMedGoogle Scholar
  • 30 Oboh G, Akinyemi AJ, Ademiluyi AO, Adefegha SA. Inhibitory effects of aqueous extract of two varieties of ginger on some key enzymes linked to type-2 diabetes in vitro . J Food Nutr Res 2010; 49: 14-20
    PubMedGoogle Scholar
  • 31 Kim JS, Kwon CS, Son KH. Inhibition of alpha-glucosidase and amylase by luteolin, a flavonoid. Biosci Biotechnol Biochem 2000; 64: 2458-2461
    CrossrefPubMedGoogle Scholar
  • 32 Matsui T, Kobayashi M, Hayashida S, Matsumoto K. Luteolin, a flavone, does not suppress postprandial glucose absorption through an inhibition of α-glucosidase action. Biosci Biotechnol Biochem 2002; 66: 689-692
    CrossrefPubMedGoogle Scholar
  • 33 De Souza Schmidt Gonçalves AE, Lajolo FM, Genovese MI. chemical composition and antioxidant/antidiabetic potential of Brazilian native fruits and commercial frozen pulps. J Agric Food Chem 2010; 58: 4666-4674
    CrossrefPubMedGoogle Scholar
  • 34 Hefny Gad M, Tuenter E, Elsawi N, Younes S, Elghadban E, Demeyer K, Pieters L, Vander Heyden Y, Mangelings D. Identification of some bioactive metabolites in a fractionated methanol extract from Ipomoea aquatica (Aerial Parts) through TLC, HPLC, UPLC-ESI-QTOF-MS and LC-SPE-NMR fingerprints analyses. Phytochem Anal 2018; 29: 5-15
    CrossrefPubMedGoogle Scholar
  • 35 Abu-Reidah IM, Arráez-Román D, Quirantes-Piné R, Fernández-Arroyo S, Segura-Carretero A, Fernández-Gutiérrez A. HPLC-ESI-Q-TOF-MS for a comprehensive characterization of bioactive phenolic compounds in cucumber whole fruit extract. Food Res Int 2012; 46: 108-117
    CrossrefPubMedGoogle Scholar
  • 36 Miyake Y, Mochizuki M, Okada M, Hiramitsu M, Morimitsu Y, Osawa T. Isolation of antioxidative phenolic glucosides from lemon juice and their suppressive effect on the expression of blood adhesion molecules. Biosci Biotech Biochem 2007; 71: 1911-1919
    CrossrefPubMedGoogle Scholar
  • 37 Marmet C, Actis-Goretta L, Renouf M, Giuffrida F. Quantification of phenolic acids and their methylates, glucuronides, sulfates and lactones metabolites in human plasma by LC-MS/MS after oral ingestion of soluble coffee. J Pharm Biomed Anal 2014; 88: 617-625
    CrossrefPubMedGoogle Scholar
  • 38 Chua LS. A review on plant-based rutin extraction methods and its pharmacological activities. J Ethnopharmacol 2013; 150: 805-817
    CrossrefPubMedGoogle Scholar
  • 39 Eum HL, Yi TG, Hong SJ, Park NI. Variations of bioactive compound contents and antioxidant capacity of asparagus seedlings in 23 varieties. Hortic Sci Technol 2020; 291-302
    PubMedGoogle Scholar
  • 40 Thiyam U, Stöckmann H, Zum Felde T, Schwarz K. Antioxidative effect of the main sinapic acid derivatives from rapeseed and mustard oil by-products. Eur J Lipid Sci Technol 2006; 108: 239-248
    CrossrefPubMedGoogle Scholar
  • 41 Boxi M, Rajesh Y, Raja V, Praveen B, Mangamma K. Extraction, phytochemical screening and in-vitro evaluation of anti-oxidant properties of Commicarpus chinensis (aqueous leaf extract). Int J Pharma Bio Sci 2010; 1: 537-547
    PubMedGoogle Scholar
  • 42 Obianime A, Uche F. The phytochemical screening and the effects of methanolic extract of Phyllanthus amarus leaf on the biochemical parameters of male guinea pigs. J Appl Sci Environ Manage 2008; 11: 73-77
    PubMedGoogle Scholar
  • 43 Sofowora A. Medicinal plants and traditional medicines in Africa. Second ed. Sunshine House, Ibadan, Nigeria: Spectrum Books; 1993: 134-156
    Google Scholar
  • 44 Trease GE, Evans WC. Pharmacognosy. Fifteenth ed. Edinburgh, London: WB Saunders; 2002: 214-393
    Google Scholar
  • 45 Yadav R, Agarwala M. Phytochemical analysis of some medicinal plants. J Phytol 2011; 3: 10-14
    PubMedGoogle Scholar
  • 46 Kim DO, Jeong SW, Lee CY. Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chem 2003; 81: 321-326
    CrossrefPubMedGoogle Scholar
  • 47 Akrout A, Gonzalez LA, El Jani H, Madrid PC. Antioxidant and antitumor activities of Artemisia campestris and Thymelaea hirsuta from southern Tunisia. Food Chem Toxicol 2011; 49: 342-347
    CrossrefPubMedGoogle Scholar
  • 48 Sharififar F, Moshafi MH, Shafazand E, Koohpayeh A. Acetyl cholinesterase inhibitory, antioxidant and cytotoxic activity of three dietary medicinal plants. Food Chem 2012; 130: 20-23
    CrossrefPubMedGoogle Scholar
  • 49 Dasgupta N, De B. Antioxidant activity of some leafy vegetables of India: a comparative study. Food Chem 2007; 101: 471-474
    CrossrefPubMedGoogle Scholar
  • 50 Khlifi D, Sghaier RM, Amouri S, Laouini D, Hamdi M, Bouajila J. Composition and anti-oxidant, anti-cancer and anti-inflammatory activities of Artemisia herba-alba, Ruta chalpensis L. and Peganum harmala L. Food Chem Toxicol 2013; 55: 202-208
    CrossrefPubMedGoogle Scholar
  • 51 Huang D, Ou B, Hampsch-Woodill M, Flanagan JA, Prior RL. High-throughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handling system coupled with a microplate fluorescence reader in 96-well format. J Agric Food Chem 2002; 50: 4437-4444
    CrossrefPubMedGoogle Scholar
  • 52 Oboh G, Akinyemi AJ, Ademiluyi AO. Inhibition of α-amylase and α-glucosidase activities by ethanolic extract of Telfairia occidentalis (fluted pumpkin) leaf. Asian Pac J Trop Biomed 2012; 2: 733-738
    CrossrefPubMedGoogle Scholar