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CC BY-NC-ND 4.0 · Planta Medica International Open 2021; 8(03): e96-e103
DOI: 10.1055/a-1494-1117
Original Papers

Screening of 20 Pantanal Wetland Plants for Anti-Candida Activity using HPLC-DAD-MS/MS and Bioautography to Characterize Active Compounds

Daniela Z. de Brito   #
1   Laboratory of Microbiological Research, Faculty of Pharmaceutical Sciences, Food and Nutrition, Federal University of Mato Grosso do Sul, Campo Grande, Brazil
,
Nadla S. Cassemiro   #
2   Laboratory of Natural Products and Mass Spectrometry - LaPNEM, Faculty of Pharmaceutical Sciences, Food and Nutrition, Federal University of Mato Grosso do Sul, Campo Grande, Brazil
,
Jeana M. E. de Souza
2   Laboratory of Natural Products and Mass Spectrometry - LaPNEM, Faculty of Pharmaceutical Sciences, Food and Nutrition, Federal University of Mato Grosso do Sul, Campo Grande, Brazil
,
Geraldo A. Damasceno-Junior
3   Laboratory of Botany, Institute of Biosciences, Federal University of Mato Grosso do Sul, Campo Grande, Brazil
,
Rodrigo J. Oliveira
4   Center for Studies and Stem Cells, Cell Therapy and Toxicological Genetics – CeTroGen, Maria Aparecida Pedrossian University Hospital, Federal University of Mato Grosso do Sul, Campo Grande, Brazil
,
Carlos A. Carollo 
2   Laboratory of Natural Products and Mass Spectrometry - LaPNEM, Faculty of Pharmaceutical Sciences, Food and Nutrition, Federal University of Mato Grosso do Sul, Campo Grande, Brazil
,
Marilene R. Chang
1   Laboratory of Microbiological Research, Faculty of Pharmaceutical Sciences, Food and Nutrition, Federal University of Mato Grosso do Sul, Campo Grande, Brazil
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Abstract

The Pantanal wetland harbors a rich flora with uncharted pharmacological potential. This study evaluated 20 Brazilian Pantanal plants against Candida albicans, C. parapsilosis, C. tropicalis, and C. krusei. Fungal susceptibility was determined by agar diffusion and broth microdilution; active compounds were identified by bioautography and HPLC-DAD-MS/MS. Sesbania virgata, Polygala molluginifolia, and Cantinoa mutabilis extracts and their chloroform and ethyl acetate (EtOAc) fractions exhibited the best activity against all Candida species tested. The EtOAc fraction of P. molluginifolia proved to be more efficient in inhibiting C. parapsilosis and C. krusei growth (Minimum inhibitory concentration of 125 and 62.5 μg/mL, respectively). Bioautography of this fraction revealed two active bands, characterized by HPLC-DAD-MS/MS as a mixture of podophyllotoxin derivatives blumenol, besides some flavonoids. This work demonstrated antifungal potential of P. molluginifolia podophyllotoxin derivatives and the versatility of bioautography with HPLC-DAD-MS/MS to identify the bioactive compounds.

Key words

Antifungal - bioautography - Candida - podophyllotoxin - Brazilian wetlands

# Daniela Z. de Brito and Nadla S. Cassemiro have equally contributed to the work and are co-authors.




Publication History

Received: 04 December 2020
Received: 20 April 2021

Accepted: 27 April 2021

Article published online:
14 July 2021

© 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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