CC BY-NC-ND 4.0 · Planta Medica International Open 2020; 07(04): e133-e144
DOI: 10.1055/a-1272-2903
Original Article

Application of UHPLC-ESI-QTOF-MS in Phytochemical Profiling of Sage (Salvia officinalis) and Rosemary (Rosmarinus officinalis)

1   Department of Physiology and Health, Maharishi International University and Health Research Institute, Fairfield, Iowa
,
Yashaswini Sharma
2   Department of Sustainable Living, Maharishi International University, Fairfield, Iowa
,
John Fagan
3   Health Research Institute, Fairfield, Iowa
,
Jim Schaefer
4   Soil Technologies Corp., Fairfield, Iowa
› Institutsangaben
Funding: Special thanks to Health Research Institute, Fairfield, Iowa for funding and providing the facility to analyze the samples
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Abstract

UHPLC with QTOF-MS is widely used as a powerful tool for metabolomic analysis. This technology has recently been applied to the analysis of polyphenols in food and herb extracts. Sage (Salvia officinalis) and rosemary (Rosmarinus officinalis), belonging to the family Lamiaceae, are known for their potent antioxidant properties due to the presence of polyphenols. We have developed a sensitive and reproducible UHPLC-QTOF-MS/MS-based method for comprehensive phytochemical profiling and the identification and quantitation of specific polyphenolic compounds present in sage and rosemary leaves. The herbs were extracted ultrasonically using methanol as the solvent. In sage, rosmarinic acid (17 678.7±673.4 µg/g) and 12-methoxy carnosic acid (21 918.3±715.4 µg/g) were found in the highest concentrations among all polyphenols. In contrast, rosmarinic acid (14 311.0±636.4 µg/g), luteolin-3'-acetyl-O-glucuronide (1488.50±47.58 µg/g), and luteolin-7-O-glucuronide (1053.68±68.83 µg/g) were observed in the highest concentrations in rosemary. Sagerinic acid, rosmanol, rosmadial, carnosol, and carnosic acid were found in abundance in both sage and rosemary. The pentacyclic triterpenoid, corosolic acid ([M - H]¯ m/z 471.35), was detected for the first time in both plants. Of the 47 polyphenolic compounds identified in each plant, 38 compounds were found in common in rosemary and sage. A flavonoid compound, baicalin ([M -H]¯ m/z 445.08), was identified for the first time in S. officinalis. Also, pectolinarigenin ([M - H]¯ m/z 313.07), a dimethoxyflavone, was detected for the first time in both sage and rosemary leaves.

Supporting Information



Publikationsverlauf

Eingereicht: 10. März 2020
Eingereicht: 14. Juli 2020

Angenommen: 24. September 2020

Artikel online veröffentlicht:
03. November 2020

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