Planta Med 2020; 86(13/14): 976-982
DOI: 10.1055/a-1091-8831
Biological and Pharmacological Activity
Original Papers

Trichothecenes from an Endophytic Fungus Alternaria sp. sb23

Ying Gao
School of Pharmacy, Tongji Medical College of Huazhong University of Science and Technology, Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, Wuhan, Peopleʼs Republic of China
,
Jia Zhou
School of Pharmacy, Tongji Medical College of Huazhong University of Science and Technology, Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, Wuhan, Peopleʼs Republic of China
,
Hanli Ruan
School of Pharmacy, Tongji Medical College of Huazhong University of Science and Technology, Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, Wuhan, Peopleʼs Republic of China
› Author Affiliations
Supported by: National Natural Science Foundation of China 31770380, 21572073, and 31270394
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Abstract

Three new (alterchothecenes A – C, 1 –3) and 3 known (4 –6) trichothecenes, along with 9 known compounds (7 –15), were isolated from the culture of Alternaria sp. sb23, an endophytic fungus separated from the root of Schisandra sphenanthera Rehd. et Wils. Their structures were elucidated by spectroscopic analyses, and the absolute configurations of 13 were determined through comparison of the experimental electronic circular dichroism (ECD) spectra and optical rotations with similar analogues. In vitro cytotoxicity tests of compounds 16 against human HT-29 colon carcinoma and human MCF-7 breast cancer cell lines indicated that 46 exhibited significant cytotoxic effects, with IC50 values ranging from 0.89 to 9.38 µM. And the potential of compounds 16 as tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) sensitizers in HT-29 cells was evaluated. The results revealed that combination treatment of TRAIL with compounds 16 synergistically decreased cell viability compared with the sole treatment with those compounds.

Supporting Information



Publication History

Received: 07 October 2019

Accepted after revision: 04 January 2020

Article published online:
04 February 2020

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