Drug Res (Stuttg) 2019; 69(12): 665-670
DOI: 10.1055/a-0986-6543
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Cytotoxic Effects of Ferula Latisecta on Human Glioma U87 Cells

Mohammad Jalili-Nik*
1   Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
,
Hamed Sabri*
1   Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
,
Ehsan Zamiri
2   Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran
,
Mohammad Soukhtanloo
1   Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
,
Mostafa Karimi Roshan
1   Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
,
Azar Hosseini
2   Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran
,
Hamid Mollazadeh
3   Department of Physiology and Pharmacology, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran
,
Mohammad Mahdi Vahedi
4   Department of Pharmacology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran
,
Amir R. Afshari
3   Department of Physiology and Pharmacology, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran
5   Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
,
Seyed Hadi Mousavi
6   Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
› Author Affiliations
Acknowledgement This work was supported by Mashhad University of Medical Sciences, Mashhad, Khorasan Razavi, Iran (grant number: 960200) Also, we appreciate all members of the Research Laboratory of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences (MUMS), for their technical support. We are very thankful to Dr. Sima Khosravi, a kind physician and a great mother for the first corresponding author (ARA) who passed away by GBM disease in fall 2016.
Further Information

Publication History

received 03 June 2019

accepted 30 July 2019

Publication Date:
09 September 2019 (online)

Abstract

Glioblastoma multiforme (GBM) is the fatal type of astrocytic tumors with a survival rate of 12 months. The present study, for the first time, evaluated the cytotoxic impacts of Ferula latisecta (F. latisecta) hydroalcoholic extract on U87 GBM cell line. The MTT assay measured the cellular toxicity following 24- and 48 h treatment with various doses of F. latisecta (0–800 μg/mL). Apoptosis was evaluated by an Annexin V/propidium iodide (PI) staining 24 h after treatment by F. latisecta. Moreover, to determine the cellular metastasis of U87 cells, we used a gelatin zymography assay (matrix metalloproteinase [MMP]-2/-9 enzymatic activity). The outcomes showed that F. latisecta mitigated the viability of U87 cells in a concentration- and time-dependent manner with IC50 values of 145.3 and 192.3 μg/mL obtained for 24- and 48 h treatments, respectively. F. latisecta induced apoptosis in a concentration-dependent manner after 24 h. Also, MMP-9 activity was significantly decreased following 24 h after treatment concentration-dependently with no change in MMP-2 enzymatic activity. This study showed that F. latisecta induced cytotoxicity and apoptosis, and mitigated metastasis of U87 GBM cells. Hence, F. latisecta could be beneficial as a promising natural herb against GBM after further studies.

* as co-first authors.


 
  • References

  • 1 Hou LC, Veeravagu A, Hsu AR. et al. Recurrent glioblastoma multiforme: A review of natural history and management options. Neurosurgical focus 2006; 20 p E3
  • 2 Omuro A. LM DeAngelis, Glioblastoma and other malignant gliomas: A clinical review. Jama 2013; 310 p 1842-1850
  • 3 Gusyatiner O, Hegi ME. Glioma epigenetics: From subclassification to novel treatment options. in Seminars in cancer biology. 2018. Elsevier; p 50-58
  • 4 Shah N, Kataria H, Kaul SC. et al. Effect of the alcoholic extract of Ashwagandha leaves and its components on proliferation, migration, and differentiation of glioblastoma cells: Combinational approach for enhanced differentiation. Cancer science 2009; 100 p 1740-1747
  • 5 Pimenov M, Leonov M. The Asian Umbelliferae biodiversity database (ASIUM) with particular reference to South-West Asian taxa. Turkish Journal of Botany 2004; 28 p 139-145
  • 6 Sahebkar A, Kataria H, Kaul SC. et al. Biological activities of essential oils from the genus Ferula (Apiaceae) 2011; p 835-847
  • 7 Habibi Z, Salehi P, Yousefi M. et al. Chemical composition and antimicrobial activity of the essential oils of Ferula latisecta and Mozaffariania insignis from Iran. Chemistry of natural compounds 2006; 42 p 689-692
  • 8 Sattar Z, Iranshahi M. Phytochemistry and pharmacology of Ferula persica Boiss.: A review. Iranian Journal of Basic Medical Sciences 2017; 20 p 1
  • 9 Mahendra P, Bisht S. Ferula asafoetida: Traditional uses and pharmacological activity. Pharmacognosy Reviews 2012; 6 p 141
  • 10 Znati M, Ben JannetH, Cazaux S. et al. Antioxidant, 5-lipoxygenase inhibitory and cytotoxic activities of compounds isolated from the Ferula lutea flowers. Molecules 2014; 19 p 16959-16975
  • 11 Znati M, Jannet HB, Cazaux S. et al. Chemical composition, biological and cytotoxic activities of plant extracts and compounds isolated from Ferula lutea. Molecules 2014; 19 p 2733-2747
  • 12 Gudarzi H, Salimi M, Irian S. et al. Ethanolic extract of Ferula gummosa is cytotoxic against cancer cells by inducing apoptosis and cell cycle arrest. Natural Product Research 2015; 29 p 546-550
  • 13 Soltani S, Amin G, Salehi-Sourmaghi MH. et al. Sulfur-containing compounds from the roots of Ferula latisecta and their cytotoxic activities. Fitoterapia 2018; 124 p 108-112
  • 14 Soltani S, Amin G-R, Salehi-Sourmaghi MH. et al. Histone deacetylase inhibitory and cytotoxic activities of the constituents from the roots of three species of Ferula. Iranian Journal of Basic Medical Sciences 2019; 22 p 93
  • 15 Sato H, Takino T, Okada Y. et al. A matrix metalloproteinase expressed on the surface of invasive tumour cells. Nature 1994; 370 p 61
  • 16 Ellis L, Canchola AJ, Spiegel D. et al. Trends in cancer survival by health insurance status in California from 1997 to 2014. JAMA oncology 2018; 4 p. 317-323
  • 17 Mollazadeh H, Afshari AR, Hosseinzadeh H. Review on the potential therapeutic roles of Nigella sativa in the treatment of patients with Cancer: Involvement of apoptosis:-Black cumin and cancer. Journal of Pharmacopuncture 2017; 20 p 158
  • 18 Boussiotis VA, Charest A. Immunotherapies for malignant glioma. Oncogene 2018; 37 p 1121
  • 19 Mollazadeh H, Boroushaki MT, Soukhtanloo M. et al. Effects of pomegranate seed oil on oxidant/antioxidant balance in heart and kidney homogenates and mitochondria of diabetic rats and high glucose-treated H9c2 cell line. Avicenna Journal of Phytomedicine 2017; 7 p 317
  • 20 Afshari AR, Roshan MK, Soukhtanloo M. et al. Investigation of cytotoxic and apoptogenic effects of terminalia chebula hydro-alcoholic extract on glioblastoma cell line. Shefaye Khatam 2018; 5 p 14-23
  • 21 Afshari AR, Roshan MK, Soukhtanloo M. et al. Cytotoxic effects of auraptene against a human malignant glioblastoma cell line. Avicenna Journal of Phytomedicine 2019; 9 p 334
  • 22 Hedge I, Lamond J, Rechinger K. Ferula, in Flora Iranica. Umbelliferae. Graz-Austria, Akademische Druck-u Verlagsanstalt 1982; p 406
  • 23 Hajimehdipoor H, Esmaeili S, Ramezani R. et al. The cytotoxic effects of Ferula persica var. persica and Ferula hezarlalehzarica against HepG2, A549, HT29, MCF7 and MDBK cell lines. Iranian Journal of Pharmaceutical Sciences 2012; 8 p 113-117
  • 24 Iranshahy M, Iranshahi M. Traditional uses, phytochemistry and pharmacology of asafoetida (Ferula assa-foetida oleo-gum-resin)—A review. Journal of Ethnopharmacology 2011; 134 p 1-10
  • 25 Rashidi R, Amiri MS, Ghorbani A. Evaluation of the effects of four plants collected from kopet-dag mountains on the proliferation of hepatocarcinoma cell line. Navid No 2019; 22 p 34-41
  • 26 Bagheri SM, Sahebkar A, Gohari AR. et al. Evaluation of cytotoxicity and anticonvulsant activity of some Iranian medicinal Ferula species. Pharmaceutical Biology 2010; 48 p 242-246
  • 27 Kasaian J, Mosaffa F, Behravan J. et al. Reversal of P-glycoprotein-mediated multidrug resistance in MCF-7/Adr cancer cells by sesquiterpene coumarins. Fitoterapia 2015; 103 p 149-154
  • 28 Mousavi SH, Tavakkol-Afshari J, Brook A. et al. Direct toxicity of Rose Bengal in MCF-7 cell line: Role of apoptosis. Food and Chemical Toxicology 2009; 47 p 855-859
  • 29 Tayarani-Najaran Z, Emami SA, Asili J. et al. Analyzing cytotoxic and apoptogenic properties of Scutellaria litwinowii root extract on cancer cell lines. Evidence-Based Complementary and Alternative Medicine 2011; 2011 p 40
  • 30 Sharifi AM, Mousavi SH, Jorjani M. Effect of chronic lead exposure on pro-apoptotic Bax and anti-apoptotic Bcl-2 protein expression in rat hippocampus in vivo. Cellular and Molecular Neurobiology 2010; 30 p 769-774
  • 31 Mousavi SH, Tayarani-Najaran Z, Hersey P. Apoptosis: From signalling pathways to therapeutic tools. Iranian Journal of Basic Medical Sciences 2008; 11 p 121-142
  • 32 Parsaee H, Asili J, Mousavi SH. et al. Apoptosis induction of salvia chorassanica root extract on human cervical cancer cell line. Iranian Journal of Pharmaceutical Research: IJPR 2013; 12 p 75
  • 33 Sharma A, Kaur M, Katnoria J. et al. Polyphenols in food: Cancer prevention and apoptosis induction. Current Medicinal Chemistry 2017; p 4740-4757
  • 34 Piperigkou Z, Manou D, Karamanou K. et al. Strategies to Target Matrix Metalloproteinases as Therapeutic Approach in Cancer, in Proteases and Cancer. 2018. Springer; p 325-348
  • 35 Sharifi AM, Mousavi SH, Farhadi M. et al. Study of high glucose-induced apoptosis in PC12 cells: role of bax protein. Journal of Pharmacological Sciences 2007; 104 p 258-262
  • 36 Asemani Y, Azadmehr A, Hajiaghaee R. et al. Anticancer potential of Ferula hezarlalehzarica Y. Ajani fraction in Raji lymphoma cell line: Induction of apoptosis, cell cycle arrest, and changes in mitochondrial membrane potential. DARU Journal of Pharmaceutical Sciences 2018; 26 p 143-154
  • 37 Moradzadeh M, Sadeghnia HR, Mousavi SH. et al. Ferula gummosa gum induces apoptosis via ROS mechanism in human leukemic cells. Cell Mol Biol (Noisy-le-grand) 2017; 63 p 17-22
  • 38 Bamehr H, Saidijam M, Dastan D. et al. Ferula pseudalliacea induces apoptosis in human colorectal cancer HCT-116 cells via mitochondria-dependent pathway. Archives of Physiology and Biochemistry 2018; p 1-8
  • 39 Kumar R, Lal N, Nemaysh V. et al. Demethoxycurcumin mediated targeting of MnSOD leading to activation of apoptotic pathway and inhibition of Akt/NF-κB survival signalling in human glioma U87 MG cells. Toxicology and Applied Pharmacology 2018; 345 p 75-93
  • 40 Xue Q, Cao L, Chen XY. et al. High expression of MMP9 in glioma affects cell proliferation and is associated with patient survival rates. Oncology Letters 2017; 13 p 1325-1330