Download PDF
Planta Med 2019; 85(11/12): 1016-1023
DOI: 10.1055/a-0955-5876
Biological and Pharmacological Activities
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Evaluation of Anti-inflammatory, Antinociceptive, and Antipyretic Activities of Prunus persica var. nucipersica (Nectarine) Kernel[ 1 ]

Abdelsamed Ibrahim Elshamy
1   Natural Compounds Chemistry Department, National Research Centre, Dokki, Giza, Egypt
,
Heba Mohammed Ibrahim Abdallah
2   Pharmacology Department, Medical Research Division, National Research Centre, Dokki, Giza, Egypt
,
Abd El-Nasser Gaber El Gendy
3   Medicinal and Aromatic Plants Research Department, National Research Centre, Dokki, Giza, Egypt
,
Walla El-Kashak
1   Natural Compounds Chemistry Department, National Research Centre, Dokki, Giza, Egypt
,
Beatrice Muscatello
4   Dipartimento di Farmacia, University of Pisa, Pisa, Italy
,
Marinella De Leo
4   Dipartimento di Farmacia, University of Pisa, Pisa, Italy
5   Centro Interdipartimentale di Ricerca “Nutraceutica e Alimentazione per la Salute”, University of Pisa, Pisa, Italy
,
Luisa Pistelli
4   Dipartimento di Farmacia, University of Pisa, Pisa, Italy
5   Centro Interdipartimentale di Ricerca “Nutraceutica e Alimentazione per la Salute”, University of Pisa, Pisa, Italy
› Author Affiliations
Further Information

Publication History

received 14 February 2019
revised 04 June 2019

accepted 05 June 2019

Publication Date:
18 June 2019 (online)

Also available at
    Permissions and Reprints

Abstract

Nectarine (Prunus persica var. nucipersica) is a worldwide appreciated edible subspecies, with a high nutritional value and benefits on human health due to its phenolic content. Despite the large consumption of the fruit, the potential use of its kernel is poorly studied. Herein, the potential pharmacological activities and the phenolic constituents of an alcoholic extract of kernel nectarine fruits were investigated. Administering nectarine kernel extract (50 and 100 mg/kg, respectively) in rats reduced paw edema after carrageenan injection by 11 and 47% in 1 h, 24 and 33% in 2 h, and 23 and 32% in 4 h, when compared to the controls. At the higher dose (100 mg/kg), nectarine kernel extract increased the reaction time in the hot-plate model and produced a significant decrease in the rectal temperature of the pyretic rats, while both doses produced 52 and 59% of writhing inhibition compared to the control group. Total polyphenolic (55.91 ± 5.78 mg/g) and flavonoid (29.89 ± 0.55 mg/g) content indicated that the extract is a promising source of these constituents. HPLC-ESI-MS/MS analysis demonstrated the presence of flavonoids, such as naringenin and apigenin glycosides. The cyanogenic glycosides amigdalin and prunasin were also detected. These results highlight the anti-inflammatory, analgesic, and antipyretic activities of nectarine kernel alcoholic extract, together with significant phenolic content, promoting its exploitation as a source of bioactive molecules.

Key words

Prunus persica var. nucipersica - Rosaceae - nectarine kernel - anti-inflammatory - analgesic - antipyretic - LC-PDA-ESI-MS/MS - flavonoids

1 Dedicated to Professor Dr. Cosimo Pizza 70th birthday in recognition of his outstanding contribution to natural product research.


 

  • References

  • 1 Siddiq M. Peach and Nectarine. In: Sinha NK, Sidhu JS, Barta J, Wu JSB, Cano MP. eds. Handbook of Fruits and Fruit Processing. Hoboken: John Wiley & Sons, Ltd.; 2012: 535-549
    CrossrefSearch in Google Scholar
  • 2 Faust M, Timon B. Origin and Dissemination of Peach. In: Janick J. ed. Horticultural Reviews. New York: Wiley; 1995: 331-379
    Search in Google Scholar
  • 3 Han W, Xu DJ, Wei XF, Zheng YD, Ma JZ, Xu XD, Wei ZG, Wang W, Zhang YC. Prokinetic activity of Prunus persica (L.) Batsch flowers extract and its possible mechanism of action in rats. Biomed Res Int 2015; 2015: 569853
    PubMedSearch in Google Scholar
  • 4 Yemane B, Medhanie G, Surender Reddy K. Survey of some common medicinal plants used in Eritrean folk medicine. Am J Ethnomed 2017; 4: 14
    PubMedSearch in Google Scholar
  • 5 Deb L, Singh RK, Singh BK, Thongam B. Some ethno-medicinal plants used by the native practitioners of Chandel district, Manipur, India. Int Res J Pharmacy 2011; 2: 199-200
    PubMedSearch in Google Scholar
  • 6 Kirtikar KR, Basu BD. Indian medicinal Plant. Dehradun: Oriental Enterprises; 2001: 1533
    Search in Google Scholar
  • 7 Sharma J, Painuli RM. Plants used for the treatment of rheumatism by the Bhoxa tribe of District Dehradun, Uttarakhand, India. Int J Med Arom Plants 2011; 1: 28-32
    PubMedSearch in Google Scholar
  • 8 Lin YR, Wu MY, Chiang JH, Yen HR, Yang ST. The utilization of traditional Chinese medicine in patients with dysfunctional uterine bleeding in Taiwan: a nationwide population-based study. BMC Complement Altern Med 2017; 17: 427
    CrossrefPubMedSearch in Google Scholar
  • 9 Aziz S, Rahman H. Biological activities of Prunus persica L. Batch. J Med Plants Res 2013; 7: 947-951
    PubMedSearch in Google Scholar
  • 10 Gasparotto J, Somensi N, Bortolin R, Moresco K, Girardi C, Klafke C, Rabelo T, Morrone M, Vizzotto M, Raseira M, Moreira J, Gelain D. Effects of different products of peach (Prunus persica L. Batsch) from a variety developed in southern Brazil on oxidative stress and inflammatory parameters in vitro and ex vivo . J Clin Biochem Nutr 2014; 55: 110-119
    CrossrefPubMedSearch in Google Scholar
  • 11 Heo M, Kim S, Yang H, Lee S, Jo H, Kim H. Protection against ultraviolet B- and C-induced DNA damage and skin carcinogenesis by the flowers of Prunus persica extract. Mutat Res 2001; 496: 47-59
    PubMedSearch in Google Scholar
  • 12 Shin T, Park S, Yoo J, Kim I, Lee H, Kwon T, Kim M, Kim J, Kim S. Anti-allergic inflammatory activity of the fruit of Prunus persica: role of calcium and NF-kappaB. Food and Chem Toxic 2010; 48: 2797-2802
    PubMedSearch in Google Scholar
  • 13 Fukuda T, Ito H, Mukainak M, Tokuda H, Nishino H, Yoshida T. Anti-tumor promoting effect of glycosides from Prunus persica seeds. Biol Pharm Bull 2003; 26: 271-273
    CrossrefPubMedSearch in Google Scholar
  • 14 Noratto G, Porter W, Byrne D, Cisneros-Zevallos L. Polyphenolics from peach (Prunus persica var. Rich Lady) inhibit tumor growth and metastasis of MDA-MB-435 breast cancer cells in vivo . J Nut Biochem 2014; 25: 796-800
    CrossrefPubMedSearch in Google Scholar
  • 15 Abidi W, Zeballos JL, Giménez R, Moreno MA, Granell A, Gogorcena Y. Bioactive compounds, sugar content and volatiles compounds in nectarine (Prunus persica (L.) Batsch) fruits. J New Sci 2015; 1: 830-835
    PubMedSearch in Google Scholar
  • 16 Nowicka P, Wojdyło A, Laskowski P. Inhibitory potential against digestive enzymes linked to obesity and type 2 diabetes and content of bioactive compounds in 20 cultivars of the peach fruit grown in Poland. Plant Foods Hum Nutr 2018; 73: 314-320
    CrossrefPubMedSearch in Google Scholar
  • 17 Rodríguez-González S, Pérez-Ramírez IF, Amaya-Cruz DM, Gallegos-Corona MA, Ramos-Gomez M, Mora O, Reynoso-Camacho R. Polyphenol-rich peach (Prunus persica L.) by-product exerts a greater beneficial effect than dietary fiber-rich by-product on insulin resistance and hepatic steatosis in obese rats. J Funct Food 2018; 45: 58-66
    CrossrefPubMedSearch in Google Scholar
  • 18 Koprivica MR, Trifković JĐ, Dramićanin AM, Gašić UM, Akšić MMF, Milojković-Opsenica DM. Determination of the phenolic profile of peach (Prunus persica L.) kernels using UHPLC-LTQ OrbiTrap MS/MS technique. Eur Food Res Technol 2018; 244: 2051-2064
    CrossrefPubMedSearch in Google Scholar
  • 19 Nowicka P, Wojdyło A. Content of bioactive compounds in the peach kernels and their antioxidant, anti-hyperglycemic, anti-aging properties. Eur Food Res Technol 2019; 245: 1123-1136
    CrossrefPubMedSearch in Google Scholar
  • 20 Wu H, Shi J, Xue S, Kakuda Y, Wang D, Jiang Y, Ye X, Li Y, Subramanian J. Essential oil extracted from peach (Prunus persica) kernel and its physicochemical and antioxidant properties. Food Sci Technol-Lebensm-Wiss Technol 2011; 44: 2032-2039
    PubMedSearch in Google Scholar
  • 21 Organization for Economical Co-operation and Development. Acute Oral Toxic Class Method Guideline 423 adopted 17.12.2001. Guidelines for the testing of chemicals Organization for Economical Co-operation and Development (OECD), Paris. Available at: https://ntp.niehs.nih.gov/iccvam/suppdocs/feddocs/oecd/oecd_gl423.pdf Accessed December 17, 2001
    PubMed
  • 22 Morebise O, Fafunso MA, Makinde JM, Olajide OA, Awe EO. Anti-inflammatory and analgesic property of leaves of Gongronem alatifolium . Phytother Res 2002; 16: S75-S77
    CrossrefPubMedSearch in Google Scholar
  • 23 Perez-Guerrero C, Herrera MD, Ortiz R, de Sotomayor MA, Fernandez MA. A pharmacological study of Cecropia obtusifolia Betrol aqueous extract. J Ethnopharmacol 2001; 76: 279-284
    CrossrefPubMedSearch in Google Scholar
  • 24 Botting RM. Inhibitors of cyclooxygenases: mechanisms, selectivity and uses. J Physiol Pharmacol 2006; 57: 113-124
    PubMedSearch in Google Scholar
  • 25 Langerman L, Zakowski MI, Piskoun B, Grant GJ. Hot plate versus tail flick: evaluation of acute tolerance to continuous morphine infusion in the rat model. J Pharmacol Toxicol Methods 1995; 34: 23-27
    CrossrefPubMedSearch in Google Scholar
  • 26 Vogel H. Drug Discovery and Evaluation: pharmacological Assays. Berlin: Springer; 2007
    Search in Google Scholar
  • 27 Raj PP. Pain Medicine: a comprehensive Review, Edition 1. Missouri: Mosby-Year Book; 1996: 12-23
    Search in Google Scholar
  • 28 Watkins LR, Milligan ED, Maier SF. Glial proinflammatory cytokines mediate exaggerated pain states: implications for clinical pain. Adv Exp Med Biol 2003; 521: 1-21
    PubMedSearch in Google Scholar
  • 29 Graham GG, Davies MJ, Day RO, Mohamudally A, Scott KF. The modern pharmacology of paracetamol: therapeutic actions, mechanism of action, metabolism, toxicity and recent pharmacological findings. Inflammopharmacology 2013; 21: 201-232
    CrossrefPubMedSearch in Google Scholar
  • 30 Senica M, Stampar F, Veberic R, Mikulic-Petkovsek M. Fruit seeds of the Rosaceae family: A waste, new Life, or a danger to human health?. J Agric Food Chem 2017; 65: 10621-10629
    CrossrefPubMedSearch in Google Scholar
  • 31 Jiménez-López J, Ruiz-Medina A, Ortega-Barralesa P, Llorent-Martínez EJ. Rosa rubiginosa and Fraxinus oxycarpa herbal teas: characterization of phytochemical profiles by liquid chromatography-mass spectrometry, and evaluation of the antioxidant activity. New J Chem 2017; 41: 7681
    CrossrefPubMedSearch in Google Scholar
  • 32 Sengupta R, Sheorey SD, Hinge MA. Analgesic and anti-inflammatory plants: an updated review. Intern J Pharm Sci Rev Res 2012; 12: 114-119
    PubMedSearch in Google Scholar
  • 33 Kim HP, Son KH, Chang HW, Kang SS. Anti-inflammatory plant flavonoids and cellular action mechanisms. J Pharmacol Sci 2004; 96: 229-245
    CrossrefPubMedSearch in Google Scholar
  • 34 Chen FF, Huo FQ, Xiong H, Wan Q, Zheng YN, Du WJ, Mei ZN. Analgesic effect of total flavonoids from Sanguis draxonis on spared nerve injury rat model of neuropathic pain. Phytomedicine 2015; 22: 1125-1132
    CrossrefPubMedSearch in Google Scholar
  • 35 Hämäläinen M, Nieminen R, Asmawi MZ, Vuorela P, Vapaatalo H, Moilanen E. Effects of flavonoids on prostaglandin E2 production. Planta Med 2011; 77: 1504-1511
    Thieme ConnectPubMedSearch in Google Scholar
  • 36 Xue N, Wu X, Wu L, Li L, Wang F. Antinociceptive and anti-inflammatory effect of naringenin in different nociceptive and inflammatory mice models. Life Sci 2019; 217: 148-154
    CrossrefPubMedSearch in Google Scholar
  • 37 El Shoubaky GA, Abdel-Daim MM, Mansour MH, Salem EA. Isolation and identification of a flavone apigenin from marine red alga Acanthophora spicifera with antinociceptive and anti-inflammatory activities. J Exp Neurosci 2016; 10: 21-29
    PubMedSearch in Google Scholar
  • 38 Hwang HJ, Kim P, Kim CJ, Lee HJ, Shim I, Yin CS, Yang Y, Hahm DH. Antinociceptive effect of amygdalin isolated from Prunus armeniaca on formalin-induced pain in rats. Biol Pharm Bull 2008; 31: 1559-1564
    CrossrefPubMedSearch in Google Scholar
  • 39 Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Amer J Enol Viticult 1965; 16: 144-158
    PubMedSearch in Google Scholar
  • 40 Dae-Ok K, Chun O, Kim YJ, Moon H, Lee CY. Quantification of polyphenolics and their antioxidant capacity in fresh plums. J Agric Food Chem 2003; 51: 6509-6515
    CrossrefPubMedSearch in Google Scholar
  • 41 Abdallah HMI, Elshamy AI, El Gendy AEG, Abd El-Gawad AM, Omer EA, De Leo M, Pistelli L. Anti-inflammatory, antipyretic, and antinociceptive effects of a Cressa cretica aqueous extract. Planta Med 2017; 83: 1313-1320
    Thieme ConnectPubMedSearch in Google Scholar
  • 42 Winter CA, Risley EA, Nuss GW. Carrageenan induced edema in hind paws of the rat as an assay for anti-inflammatory drugs. Proc Soc Exp Biol Med 1962; 111: 544-547
    CrossrefPubMedSearch in Google Scholar
  • 43 Ammar NM, El-Hawary SS, El-Anssary AA, El-Desoky AH, Abdelaal TA, Abdelrahman RF, Hattori M. Phytochemical study of the bioactive fractions of Chrysanthemum fructescens L. cultivated in Egypt. Int J Pharmacognosy Phytochem Res 2016; 8: 1314-1321
    PubMedSearch in Google Scholar
  • 44 Woolfe CJ, McDonald AD. The evaluation of the analgesic action of pethidine hydrochloride (demerol). J Pharmacol Exp Ther 1962; 80: 300-307
    PubMedSearch in Google Scholar
  • 45 Laviola G, Alleva E. Ontogeny of muscimol effects on locomotor activity, habituation and pain reactivity in mice. Psychopharmacology (Berl) 1990; 102: 41-48
    CrossrefPubMedSearch in Google Scholar
  • 46 Koster R, Anderson M, de Beer EJ. Acetic acid for analgesic screening. Fed Proc 1959; 18: 412-416
    PubMedSearch in Google Scholar
  • 47 Vogel HG. Antipyretic Activity. In: Vogel HG. ed. Drug Discovery and Evaluation: pharmacological Assays. New York: Springer; 2002: 418-420
    Search in Google Scholar
  • 48 Manivel K, Rajangam P, Muthusamy K, Rajasekar S. Evaluation of anti-pyretic effect of Trichosanthes tricuspidata Linn on albino rats. Int J Res Pharm Biomed Sci 2011; 2: 1718-1720
    PubMedSearch in Google Scholar