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CC BY-NC-ND 4.0 · Planta Medica International Open 2019; 6(01): e7-e14
DOI: 10.1055/a-0829-0454
Original Papers
Eigentümer und Copyright ©Georg Thieme Verlag KG 2019

Effect of the Extract and Constituents From Hancornia speciosa Fruits in Osteoclasts

Micena Roberta Miranda Alves e Silva   *
1   Department of Morphology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
,
Cinthia Mara da Fonseca Pacheco   *
2   Dental School, Centro Universitário Newton Paiva, Belo Horizonte, MG, Brazil
,
Mila Fernandes Moreira Madeira
3   Department of Microbiology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
,
Adriana Machado Saraiva
4   Department of Oral Surgery and Oral Pathology, Faculty of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
,
Elisângela Alves de Freitas
5   Universidade Federal de Lavras, Lavras, MG, Brazil
,
Thalita Marcolan Valverde
6   Department of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
,
José Hugo de Sousa Gomes
7   Department of Pharmaceutical Products, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
,
Rodrigo Maia de Pádua
7   Department of Pharmaceutical Products, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
,
Gregory Thomas Kitten
1   Department of Morphology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
,
Sandra Y.Fukada Alves
8   Department of Pharmacological Science, Faculty of Pharmacy, University of São Paulo, São Paulo, SP, Brazil
,
Fernão Castro Braga
7   Department of Pharmaceutical Products, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
,
Tarcília Aparecida da Silva
4   Department of Oral Surgery and Oral Pathology, Faculty of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
› Author Affiliations
Further Information

Publication History

received 14 August 2018
revised 02 December 2018

accepted 06 December 2018

Publication Date:
05 February 2019 (online)

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Abstract

Hancornia speciosa is a medicinal species traditionally used in Brazilian folk medicine to treat a variety of conditions. Compounds isolated from the leaves, bark, and trunk of this plant have shown therapeutic properties, but only recently have the fruits of H. speciosa been explored for potential pharmacological applications. The present study investigated the effects of an ethanolic extract from the fruits, fractions, and compounds thereof in bone resorbing cells. Primary osteoclast cultures from bone marrow cells and osteoclasts derived from a monocyte/macrophage cell line, RAW 264.7, were incubated with different concentrations of the ethanolic extract, ethyl acetate fraction, water fraction, quinic acid, and L-(+)-bornesitol. In RAW 264.7 cell cultures, quinic acid significantly reduced osteoclast formation. In bone marrow cell-derived osteoclasts, the ethyl acetate fraction induced a decrease in the number of osteoclasts, promoting a remarkable reduction in the mean area of those cells and in their resorption activity. The compounds quinic acid and bornesitol also affected bone marrow cell-derived osteoclasts. In both cell cultures, the substances tested did not affect cell viability/proliferation. In conclusion, components extracted from H. speciosa fruit affected the cells responsible for bone resorption, making them promising tools for interference in osteoclastogenesis.

Key words

Hancornia speciosa - Apocynaceae - cell culture - osteoclasts - bone resorption

* These authors contributed equally to the work presented in this paper.


Supporting Information

 

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