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Planta Med 2019; 85(07): 563-569
DOI: 10.1055/a-0725-8456
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Rosehip Oil Promotes Excisional Wound Healing by Accelerating the Phenotypic Transition of Macrophages

Zhiyong Lei
1   Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China
,
Zhijian Cao
2   Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology and Center for Human Genome Research, Huazhong University of Science and Technology, Wuhan, China
,
Zaiwang Yang
1   Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China
,
Mingzhang Ao
1   Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China
,
Wenwen Jin
1   Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China
,
Longjiang Yu
1   Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China
› Author Affiliations
Further Information

Publication History

received 03 June 2018
revised 18 August 2018

accepted 24 August 2018

Publication Date:
10 September 2018 (online)

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Abstract

Poor wound healing is a major and global threat to public health. Efforts have been made to better understand the underlying mechanisms and develop effective remedies, though the advancements that have been made are still limited. As there are no effective and generally applicable therapies available for skin injuries and fibrosis, it is urgent to develop new drugs and therapies that facilitate wound healing and effectively improve scars. In this study, GC-MS analysis was performed to identify the chemical composition of rosehip oil. The excisional wound healing model and the carrageenan-induced paw edema method were respectively applied to evaluate the wound healing activity and anti-inflammatory activity of rosehip oil. Hematoxylin and eosin staining was used to assess the pathological changes of sections, and Sirius-red staining was performed to analyze the ratio of collagen I/III in wound tissues. Immunohistological staining for CD68, CCR7 (CD197), CD163, TGF-β1, and α-SMA was applied to determine the macrophage phenotypes transition (M1-to-M2) and demonstrate the scar-improving efficacy of rosehip oil on wound healing. Results showed that rosehip oil significantly promoted wound healing and effectively improved scars. This efficacy might be exerted by accelerating the macrophage phenotypes transition and inhibiting the process of epithelial-mesenchymal transition.

Key words

wound healing - excisional wound - anti-inflammatory activity - rosehip oil - Rosa canina - Rosaceae

Supporting Information

    A GC-MS chromatogram of RO and pictures of wounds taken on days 0, 2, 5, 7, 10, 14, and 21 are available as Supporting Information.

  • Supporting Information
 

  • References

  • 1 Robson MC, Steed DL, Franz MG. Wound healing: biologic features and approaches to maximize healing trajectories. Curr Probl Surg 2001; 38: 72-140
    PubMedSearch in Google Scholar
  • 2 Weiser TG, Regenbogen SE, Thompson KD, Haynes AB, Lipsitz SR, Berry WR, Gawande AA. An estimation of the global volume of surgery: a modelling strategy based on available data. Lancet 2008; 372: 139-144
    CrossrefPubMedSearch in Google Scholar
  • 3 Erickson JR, Echeverri K. Learning from regeneration research organisms: The circuitous road to scar free wound healing. Dev Biol 2018; 433: 144-154
    CrossrefPubMedSearch in Google Scholar
  • 4 Vision Gain. Advanced wound care: world market prospects 2011–2021. Available at: http://www.visiongain.com/Report/716/Advanced-Wound-Care-World-Market-Prospects-2011-2021 Accessed November 15, 2011
    PubMed
  • 5 Sen CK, Gordillo GM, Roy S, Kirsner R, Lambert L, Hunt TK, Gottrup F, Gurtner GC, Longaker MT. Human skin wounds: a major and snowballing threat to public health and the economy. Wound Rep Reg 2009; 17: 763-771
    CrossrefPubMedSearch in Google Scholar
  • 6 Mukherjee H, Ojha D, Bharitkar YP, Ghosh S, Mondal S, Kaity S, Dutta S, Samanta A, Chatterjee TK, Chakrabarti S, Mondal NB, Chattopadhyay D. Evaluation of the wound healing activity of Shorea robusta, an Indian ethnomedicine, and its isolated constituent(s) in topical formulation. J Ethnopharmacol 2013; 149: 335-343
    CrossrefPubMedSearch in Google Scholar
  • 7 Kasuya A, Tokura Y. Attempts to accelerate wound healing. J Dermatol Sci 2014; 76: 169-172
    CrossrefPubMedSearch in Google Scholar
  • 8 Zomer HD, Trentin AG. Skin wound healing in humans and mice: Challenges in translational research. J Dermatol Sci 2018; 90: 3-12
    CrossrefPubMedSearch in Google Scholar
  • 9 Miller MC, Nanchahal J. Advances in the modulation of cutaneous wound healing and scarring. BioDrugs 2005; 19: 363-381
    CrossrefPubMedSearch in Google Scholar
  • 10 Behm B, Babilas P, Landthaler M, Schreml S. Cytokines, chemokines and growth factors in wound healing. J Eur Acad Dermatol 2012; 26: 812-820
    CrossrefPubMedSearch in Google Scholar
  • 11 Bielefeld KA, Amini-Nik S, Alman BA. Cutaneous wound healing: recruiting developmental pathways for regeneration. Cell Mol Life Sci 2013; 70: 2059-2081
    CrossrefPubMedSearch in Google Scholar
  • 12 Guo S, DiPietro LA. Factors affecting wound healing. J Dent Res 2010; 89: 219-229
    CrossrefPubMedSearch in Google Scholar
  • 13 Udupa AL, Kulkarni DR, Udupa SL. Effect of Tridax procumbens extracts on wound healing. Int J Pharmacogn 1995; 33: 37-40
    PubMedSearch in Google Scholar
  • 14 Perez JL, Rohrich RJ. Optimizing postsurgical scars: a systematic review on best practices in preventative scar management. Plast Reconstr Surg 2017; 140: 782e-793e
    CrossrefPubMedSearch in Google Scholar
  • 15 Das U, Behera SS, Pramanik K. Ethno-herbal-medico in wound repair: an incisive review. Phytother Res 2017; 31: 579-590
    CrossrefPubMedSearch in Google Scholar
  • 16 Wedler J, Daubitz T, Schlotterbeck G, Butterweck V. In vitro anti-inflammatory and wound-healing potential of a Phyllostachys edulis leaf extract-identification of isoorientin as an active compound. Planta Med 2014; 80: 1678-1684
    Thieme ConnectPubMedSearch in Google Scholar
  • 17 Gautam MK, Gangwar M, Singh SK, Goel RK. Effects of Azardirachta indica on vascular endothelial growth factor and cytokines in diabetic deep wound. Planta Med 2015; 81: 713-721
    Thieme ConnectPubMedSearch in Google Scholar
  • 18 Verjee S, Garo E, Pelaez S, Fertig O, Hamburger M, Butterweck V. Saffron flower extract promotes scratch wound closure of keratinocytes and enhances VEGF production. Planta Med 2017; 83: 1176-1183
    Thieme ConnectPubMedSearch in Google Scholar
  • 19 Ilyasoğlu H. Characterization of rosehip (Rosa canina L.) seed and seed oil. Int J Food Prop 2014; 17: 1591-1598
    CrossrefPubMedSearch in Google Scholar
  • 20 Osojnik Črnivec IG, Muri P, Djinović P, Pintar A. Biogas production from spent rose hips (Rosa canina L.): fraction separation, organic loading and co-digestion with N-rich microbial biomass. Bioresour Technol 2014; 171: 375-383
    CrossrefPubMedSearch in Google Scholar
  • 21 Paladines D, Valero D, Valverde JM, Díaz-Mula H, Serrano M, Martínez-Romero D. The addition of rosehip oil improves the beneficial effect of Aloe vera gel on delaying ripening and maintaining postharvest quality of several stonefruit. Postharvest Biol Tec 2014; 92: 23-28
    CrossrefPubMedSearch in Google Scholar
  • 22 Zhao G, Etherton TD, Martin KR, Vanden Heuvel JP, Gillies PJ, West SG, Kris-Etherton PM. Anti-inflammatory effects of polyunsaturated fatty acids in THP-1 cells. Biochem Biophys Res Commun 2005; 336: 909-917
    CrossrefPubMedSearch in Google Scholar
  • 23 Delmastro-Greenwood M, Freeman BA, Wendell SG. Redox-dependent anti-inflammatory signaling actions of unsaturated fatty acids. Annu Rev Physiol 2014; 76: 79-105
    CrossrefPubMedSearch in Google Scholar
  • 24 Nissinen LM, Kahari VM. Collagen turnover in wound repair – a macrophage connection. J Invest Dermatol 2015; 135: 2350-2352
    CrossrefPubMedSearch in Google Scholar
  • 25 Zhu Z, Ding J, Tredget EE. The molecular basis of hypertrophic scars. Burns Trauma 2016; 4: 2
    PubMedSearch in Google Scholar
  • 26 Moore KW, de Waal MR, Coffman RL, OʼGarra A. Interleukin-10 and the interleukin-10 receptor. Annu Rev Immunol 2001; 19: 683-765
    CrossrefPubMedSearch in Google Scholar
  • 27 Sindrilaru A, Peters T, Wieschalka S, Baican C, Baican A, Peter H, Hainzl A, Schatz S, Qi Y, Schlecht A, Weiss JM, Wlaschek M, Sunderkötter C, Scharffetter-Kochanek K. An unrestrained proinflammatory M1 macrophage population induced by iron impairs wound healing in humans and mice. J Clin Invest 2011; 121: 985-997
    CrossrefPubMedSearch in Google Scholar
  • 28 Mirza RE, Fang MM, Novak ML, Urao N, Sui A, Ennis WJ, Koh TJ. Macrophage PPARγ and impaired wound healing in type 2 diabetes. J Pathol 2015; 236: 433-444
    CrossrefPubMedSearch in Google Scholar
  • 29 Khanna S, Biswas S, Shang Y, Collard E, Azad A, Kauh C, Bhasker V, Gordillo GM, Sen CK, Roy S. Macrophage dysfunction impairs resolution of inflammation in the wounds of diabetic mice. PLoS One 2010; 5: e9539
    CrossrefPubMedSearch in Google Scholar
  • 30 Mueller CK, Schultze-Mosgau S. Histomorphometric analysis of the phenotypical differentiation of recruited macrophages following subcutaneous implantation of an allogenous acellular dermal matrix. Int J Oral Max Surg 2011; 40: 401-407
    CrossrefPubMedSearch in Google Scholar
  • 31 Manduch M, Dexter DF, Jalink DW, Vanner SJ, Hurlbut DJ. Undifferentiated pancreatic carcinoma with osteoclast-like giant cells: report of a case with osteochondroid differentiation. Pathol Res Pract 2009; 205: 353-359
    CrossrefPubMedSearch in Google Scholar
  • 32 Hopken UE, Winter S, Achtman AH, Kruger K, Lipp M. CCR7 regulates lymphocyte egress and recirculation through body cavities. J Leukoc Biol 2010; 87: 671-682
    CrossrefPubMedSearch in Google Scholar
  • 33 Siebert JW, Burd AR, McCarthy JG, Weinzweig J, Ehrlich HP. Fetal wound healing: a biochemical study of scarless healing. Plast Reconstr Surg 1990; 85: 495-502
    CrossrefPubMedSearch in Google Scholar
  • 34 Merkel JR, DiPaolo BR, Hallock GG, Rice DC. Type I and type III collagen content of healing wounds in fetal and adult rats. Exp Biol Med 1988; 187: 493-497
    CrossrefPubMedSearch in Google Scholar
  • 35 Hallock GG, Ricem DC, Merkel JR, DiPaolo BR. Analysis of collagen content in the fetal wound. Ann Plast Surg 1988; 21: 310-315
    CrossrefPubMedSearch in Google Scholar
  • 36 Cameron AM, Adams DH, Greenwood JE, Anderson PJ, Cowin AJ. A novel murine model of hypertrophic scarring using subcutaneous infusion of bleomycin. Plast Reconstr Surg 2014; 133: 69-78
    CrossrefPubMedSearch in Google Scholar
  • 37 Tomasek JJ, Gabbiani G, Hinz B, Chaponnier C, Brown RA. Myofibroblasts and mechano-regulation of connective tissue remodelling. Nat Rev Mol Cell Bio 2002; 3: 349-363
    CrossrefPubMedSearch in Google Scholar
  • 38 Van De Water L, Varney S, Tomasek JJ. Mechanoregulation of the myofibroblast in wound contraction, scarring, and fibrosis: opportunities for new therapeutic intervention. Adv Wound Care 2013; 2: 122-141
    CrossrefPubMedSearch in Google Scholar
  • 39 Pohlers D, Brenmoehl J, Löffler I, Müller CK, Leipner C, Schultze-Mosgau S, Stallmach A, Kinne RW, Wolf G. TGF-β and fibrosis in different organs – molecular pathway imprints. Biochim Biophys Acta 2009; 1792: 746-756
    PubMedSearch in Google Scholar
  • 40 FDA. 2006 Guidance for industry: chronic cutaneous ulcer and burn wounds-developing products for treatment. Official publication of the US Department of Health and Human Services – Food and Drug Administration (FDA) – Center for Drug Evaluation and Research (CDER) – Center for Biologics Evaluation and Research (CBER) – Center for Devices and Radiological Health (CDRH) pp. 18. Available at at: http://www.fda.gov/cber/gdlns/ulcburn.htm Accessed June 1, 2006
    PubMed
  • 41 Mu Y, Xu Z, Zhou X, Zhang H, Yang Q, Zhang Y, Xie Y, Kang J, Li F, Wang S. 2,3,5,4-Tetrahydroxystilbene-2-O-β-D-glucoside attenuates ischemia/reperfusion-induced brain injury in rats by promoting angiogenesis. Planta Med 2017; 83: 676-683
    Thieme ConnectPubMedSearch in Google Scholar