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DOI: 10.1055/a-0690-9236
Lanostane Triterpenes from Gloeophyllum odoratum and Their Anti-Influenza Effects
Publikationsverlauf
received 05. Juli 2018
revised 08. August 2018
accepted 14. August 2018
Publikationsdatum:
21. August 2018 (online)
![](https://www.thieme-connect.de/media/plantamedica/201903/lookinside/thumbnails/10-1055-a-0690-9236_pmc0528-1.jpg)
Abstract
In an in vitro screening for anti-influenza agents from European polypores, the fruit body extract of Gloeophyllum odoratum dose-dependently inhibited the cytopathic effect of the H3N2 influenza virus A/Hong Kong/68 (HK/68) in Madin Darby canine kidney cells with a 50% inhibitory concentration (IC50) of 15 µg/mL, a noncytotoxic concentration. After a chromatographic work-up, eight lanostane triterpenes (1–8) were isolated and their structures were elucidated based on high-resolution electrospray ionization mass spectrometry analyses, and one- and two-dimensional nuclear magnetic resonance experiments. Constituents 1 (gloeophyllin K) and 2 (gloeophyllin L) are reported here for the first time, and compounds 5, 7, and 8 have not been described for the investigated fungal material so far. The highest activity was determined for trametenolic acid B (3) against HK/68 and the 2009 pandemic H1N1 strain A/Jena/8178/09 with IC50 values of 14 and 11 µM, respectively. In a plaque reduction assay, this compound was able to bind to cell-free viruses and to neutralize their infectivity.
Key words
Gloeophyllum odoratum - Gloeophyllales - polypores - lanostane triterpenes - anti-influenzaSupporting Information
- Supporting Information
The NMR, HRESIMS, and IR spectra of compounds 1 and 2 and graphs showing dose-dependencies for the investigated compounds (Fig. 24S) are provided as Supporting Information (Fig. 1S–23S).
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