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Exp Clin Endocrinol Diabetes 2019; 127(06): 396-404
DOI: 10.1055/a-0659-9928
Article
© Georg Thieme Verlag KG Stuttgart · New York

Acute Administration of Tibolone Prevents Oxidative Stress in Ovariectomized Rats Fed High-Fat-and-Fructose Diet

Norma Angélica Estrada Cruz
1   Unidad de Investigación Médica en Farmacología, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico
2   Posgrado en Biología Experimental, División en Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana Iztapalapa, Mexico City, Mexico
,
Julio César Almanza Pérez
3   Laboratorio de Farmacología, Departamento de Ciencias de la Salud, División en Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana Iztapalapa, Mexico City, Mexico
,
Ángeles Fortis Barrera
3   Laboratorio de Farmacología, Departamento de Ciencias de la Salud, División en Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana Iztapalapa, Mexico City, Mexico
,
Juan Manuel Gallardo
4   Unidad de Investigación Médica en Enfermedades Nefrológicas, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico
,
Leticia Manuel Apolinar
5   Unidad de Investigación Médica en Enfermedades Endocrinas, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico
,
Julia Jeanett Segura Uribe
6   Unidad de Investigación Médica en Enfermedades Neurológicas, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico
,
Sandra Orozco Suárez
6   Unidad de Investigación Médica en Enfermedades Neurológicas, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico
,
Angélica Coyoy Salgado
7   CONACyT-Unidad de Investigación Médica en Enfermedades Neurológicas, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico
,
Christian Guerra Araiza
1   Unidad de Investigación Médica en Farmacología, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Mexico City, Mexico
› Author Affiliations
Further Information

Publication History

Received 11 April 2018
Revised 14 June 2018

Accepted 17 July 2018

Publication Date:
14 August 2018 (online)

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Abstract

In addition to oxidative stress due to the increase of free radicals, estrogen deficiency is associated with changes in enzymatic activity, glutathione redox ratio (GSH/GSSG), and the content of oxidative markers such as malondialdehyde. Tibolone, a synthetic steroid, has been used as an elective treatment for the relief of menopausal symptoms. However, the acute effects of hormonal therapy with tibolone on metabolic parameters and oxidative stress markers associated with the first stages of estrogen deficiency are still unknown. The study aimed to evaluate if the acute administration of tibolone reduces oxidative stress in ovariectomized rats fed high-fat-and-fructose diet. Rats were fed a standard diet or a diet consisting of 10% lard-supplemented chow and 20% high-fructose syrup in the drinking water plus tibolone or vehicle for seven days. Weight, cholesterol, triglycerides, and glucose levels, as well as antioxidant enzymes and oxidative stress markers were quantified in the serum of each experimental group. It was observed that seven days of diet and tibolone treatment in the ovariectomized group reduced weight, triglycerides, cholesterol, glucose levels and advanced glycation end products but did not change GSH/GSSG ratio nor the enzymatic activity of superoxide dismutase. Also, both glutathione peroxidase and glutathione reductase activity decreased, as well as malondialdehyde levels. These results suggest that the acute treatment with tibolone prevented the changes in the metabolic parameters analyzed as well as the increase in the levels of malondialdehyde and AGEs induced by ovariectomy and high-fat diet.

Key words

hormonal therapy - estrogen deficiency - glutathione - malondialdehyde - advanced glycation end products
 

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