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DOI: 10.1055/a-0626-7356
Anti-inflammatory Flavanones and Flavanols from the Roots of Pongamia pinnata
Publication History
received 22 November 2017
revised 16 April 2018
accepted 27 April 2018
Publication Date:
18 May 2018 (online)


Abstract
A phytochemical study of the roots of Pongamia pinnata afforded 29 flavanones and flavanols, including 7 previously undescribed compounds. The structures of the isolated compounds were determined by 1D and 2D NMR and mass spectroscopy data. The absolute configurations of the compounds were assigned via analysis of the specific rotations and electronic circular dichroism spectra, application of Mosherʼs method, and by comparing the calculated and experimental electronic circular dichroism spectra. The isolates were evaluated for their inhibitory effects on nitric oxide production in lipopolysaccharide-stimulated BV-2 microglial cells. All of the isolated compounds exhibited inhibitory effects against nitric oxide production, and most of them showed obvious anti-inflammatory activities (IC50 < 20 µM), among which 26 was the most active compound with an IC50 of 9.6 µM.
Key words
Pongamia pinnata - Leguminosae - flavanones - flavanols - anti-inflammatory effect - nitric oxide inhibitionSupporting Information
- Supporting Information
HRESIMS and NMR spectra of compounds 1–7 (Figs. 1S–49S), NMR data of compounds 8, 11, 22, 24, 26, 28, and 29 (Tables 1S–7S), CD spectra of compounds 4, 5, and 29 (Fig. 50S), and the dose-response column graphs of the active compounds (Fig. 51S) are available as Supporting Information.