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Homeopathy 2009; 98(03): 160-164
DOI: 10.1016/j.homp.2009.05.005
Original Paper
Copyright © The Faculty of Homeopathy 2009

Effect of Mercurius solubilis on the bacteriological response in the alveolitis process in rats

Flávia Regina Gonçalves de Araújo
1   Federal University of Pernambuco, Microbiology Division, Laboratory of Immunopathology Keizo-Asami, UFPE, Brazil
,
Célia Maria Machado Barbosa de Castro
2   Department of Tropical Medicine, Laboratory of Immunopathology Keizo-Asami, UFPE, Brazil
,
Maiara Santos Severo
3   Microbiology Division, Laboratory of Immunopathology Keizo-Asami, UFPE, Brazil
,
Maria de Fátima Alves Diniz
3   Microbiology Division, Laboratory of Immunopathology Keizo-Asami, UFPE, Brazil
,
Marcelo Tavares Viana
3   Microbiology Division, Laboratory of Immunopathology Keizo-Asami, UFPE, Brazil
,
Liriane Baratella Evêncio
4   Department of Histology, Laboratory of Immunopathology Keizo-Asami, UFPE, Brazil
› Author Affiliations
Subject Editor:
Further Information

Publication History

Received06 May 2008
revised07 May 2009

accepted19 May 2009

Publication Date:
22 December 2017 (online)

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Objective: The purpose of this study was to assess the bacteriological response in alveolitis in rats treated with the homeopathic medicine Merc solubilis (Merc sol.) 12 cH.

Methods: The study was randomized and observer blind. The animals were anesthetized and the upper right incisor extracted resulting in alveolitis. Animals were randomly assigned to groups (n = 18/group): Water control, Alcohol control and Merc sol. 12 cH. These groups were subsequently divided into 3 subgroups (n = 6/subgroup): Early Euthanasia (EE), Mid Euthanasia (ME) and Late Euthanasia (LE), killed at the 6th, 15th and 21st days respectively. The perialveolar microbiota was collected by swab in Brain Heart Infusion (BHI) for seeding and bacterioscopy. After seeding, the Petri dishes were incubated at 37°C for 48 h.

Results: Quantitative and qualitative changes were observed in the perialveolar microbiota when the groups were compared. Water control and Alcohol control had the highest counts of pathogenic bacteria, the microbiotica of the Merc sol. group remained closer to normal.

Conclusions: Merc sol. 12 cH did not reduce bacterial growth, but the microbiotica remained within the parameters of normality, obtaining the best results at 21 days after treatment.

Keywords

Merc solurius solubilis - alveolitis - bacteria - perialveolar microbiota
 

  • References

  • 1 Neville B.W., Damm D.D., Allen C.M., Bouquot J.E. Doenças da polpa e do periápice. Patologia Oral e Maxilofacial. 2nd edn. c 2004. Rio de Janeiro: Guanabara Koogan; 105-132
    Google Scholar
  • 2 Torres-Lagares D., Serrera-Figallo M.A., Romero-Ruíz M.M., Infante-Cossío P., García-Calderón M., Gutiérrez-Perez J.L. Alveolitis seca: Actualización de conceptos. Med Oral Patol Oral Cir Bucal 2005; 10: 77-85.
    PubMedGoogle Scholar
  • 3 Arteagoitia-Calvo M.I., Diez-Garcia M.A., Barbier-Herrero L., Landa-Liona S., Santamaría-Arrieta G., Santamaría-Zuazua J. Antibioterapia sistémica preventiva de la alveolitis seca en la exodoncia del tercer molar inferior: revisión sistemática. RCOE 2002; 7: 603-611.
    PubMedGoogle Scholar
  • 4 Caso A., Hung L.K., Beirne O.R. Prevention of alveolar osteitis with chlorhexidine: a meta-analytic review. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2005; 99: 155-159.
    CrossrefPubMedGoogle Scholar
  • 5 Tortora G.J., Funke B.R., Case C.L. Princípios de doença e epidemiologia. In: Microbiologia. 6th edn. 2000. Porto Alegre: Artes Médicas Sul; 394-420
    Google Scholar
  • 6 Ye P., Harty D.W.S., Chapple C.C., Nadkarni M.A., Carlo A.A.D.E., Hunter N. Streptococci and Actinomyces induce antibodies which cross react with epithelial antigens in periodontitis. Clin Exp Immunol 2003; 131: 468-476.
    CrossrefPubMedGoogle Scholar
  • 7 Vickers A.J., Van Haselen R.A., Pang L., Berkovitz S. Inter-rater reliability of symptom repertorisation: a pragmatic empirical study. Br Hom J 2000; 89: 188-191.
    CrossrefPubMedGoogle Scholar
  • 8 Lathoud J.A. Merc solurius vivus. In: Estudos de Matéria Médica Homeopática. 2001. São Paulo: Organon; 729-743
    Google Scholar
  • 9 Ribeiro Filho A. Novo Repertório de Sintomas Homeopáticos. São Paulo: Robe; 1996. 1201 pp
    Google Scholar
  • 10 Okamoto T., Russo M.C. Wound healing following tooth extraction. Histochemical study in rats. Rev Odont Araçatuba 1973; 2: 153-163.
    PubMedGoogle Scholar
  • 11 Corrêa A.D., Siqueira-Batista R., Quintas L.E.M. Similia similibus curentur: notação histórica da medicina homeopática. Rev Assoc Med Brasil 1997; 43: 347-351.
    PubMedGoogle Scholar
  • 12 Marcotte H., Lavoie M.C. Oral microbial ecology and the role of salivary immunoglobulin A. Microbiol Mol Biol Rev 1998; 62: 71-109.
    PubMedGoogle Scholar
  • 13 Leyva A.L.A., Paneque M.E. Homeopatía. Rev Cubana Med Gen Integr 1997; 13: 320-324.
    PubMedGoogle Scholar
  • 14 Paster B.J., Boches S.K., Galvin J.L. et al. Bacterial diversity in human subgingival plaque. J Bacteriol 2001; 183: 3770-3783.
    CrossrefPubMedGoogle Scholar
  • 15 Koller M.M., Maeda N., Scarpace P.J., Humphreys-Beher M.G. Desipramine changes salivary gland function, oral microbiota, and oral health in rats. Eur J Pharmacol 2000; 408: 91-98.
    CrossrefPubMedGoogle Scholar
  • 16 Pelczar Júnior M.J., Chan E.C.S., Krieg N.R. Flora normal do corpo humano. In: Microbiologia Conceitos e Aplicações. 2nd edn. 1996. São Paulo: Makron Books; 22-40
    Google Scholar
  • 17 Chow A.W. Infections of the oral cavity, neck, and head. In: Mandell G.L., Bennett J.E., Dolin R. Principles and Practice of Infectious Diseases. 5th edn. 2000. Philadelphia: Churchill Livingstone; 689-701
    Google Scholar
  • 18 Darby I., Curtis M. Microbiology of periodontal disease in children and young adults. Periodontol 2000 2001; 26: 33-53.
    CrossrefPubMedGoogle Scholar
  • 19 Santos S.S.F., Jorge A.O.C. Sensibilidade “in vitro” de enterobacteriaceae e pseudomonadaceae isoladas da cavidade bucal humana a agentes antimicrobianos. Pós-Grad Rev 1999; 2: 41-44.
    PubMedGoogle Scholar
  • 20 Nishihara T., Koseki T. Microbial etiology of periodontitis. Periodontol 2000 2004; 36: 14-26.
    CrossrefPubMedGoogle Scholar
  • 21 Roberts F.A., Darveau R.P. Beneficial bacteria of the periodontium. Periodontol 2000 2002; 30: 40-50.
    CrossrefPubMedGoogle Scholar
  • 22 Weinberg M.A., Bral M. Laboratory animal models in periodontology. J Clin Periodontol 1999; 26: 335-340.
    CrossrefPubMedGoogle Scholar
  • 23 Beem J.E., Hurley C.G., Magnusson I., McArthur W.P., Clark W.B. Subgingival microbiota in squirrel monkeys with naturally occurring periodontal diseases. Infect Immun 1991; 59: 4034-4041.
    PubMedGoogle Scholar
  • 24 Rodrigues A.M., Newman M.G. Microbiologia e imunologia periodontal. In: Cardoso R.J.A., Gonçalves E.A.N. Periodontia/Cirurgia/Cirurgia para implantes. 2002. São Paulo: Artes Médicas; 21-50
    Google Scholar
  • 25 Socransky S.S., Haffajee A.D. Periodontal microbial ecology. Periodontol 2000 2005; 38: 135-187.
    CrossrefPubMedGoogle Scholar
  • 26 Ximenez-Fyvie L.A., Haffajee A.D., Socransky S.S. Comparison of the microbiota of supra and subgingival plaque in health and periodontitis. J Clin Periodontol 2000; 27: 648-657.
    CrossrefPubMedGoogle Scholar
  • 27 D'Antônio GM. Contaminação pós-exodôntica do alvéolo dental de ratos. Estudo microbiológico e histológico, 1984, 52 f. Tese (Doutorado) – Faculdade de Odontologia, Universidade Estadual Paulista, Araçatuba.
    PubMed
  • 28 Melo Júnior E.J.M., Raposo M.J., Sant'ana A.E.G., Lisboa Neto J.A., Diniz M.F.A. Estudo de plantas medicinais com atividade antimicrobiana sobre microrganismos presentes na alveolite. Rev ABO Nac 2000; 8: 220-226.
    PubMedGoogle Scholar
  • 29 Gutiérrez-Pérez J.L., Perea-Pérez E.J., Romero-Ruiz M.M., Girón-González J.A. Infecciones orofaciales de origen odontogénico. Med Oral 2004; 9: 280-287.
    PubMedGoogle Scholar
  • 30 Haffajee A.D., Socransky S.S. Microbiology of periodontal diseases: introduction. Periodontol 2000 2005; 38: 9-12.
    CrossrefPubMedGoogle Scholar
  • 31 Koller M.M., Purushotham K.R., Maeda N., Scarpace P.J., Humphreys-Beher M.G. Desipramine induced changes in salivary proteins, cultivable oral microbiota and gingival health in aging female NIA Fischer 344 rats. Life Sci 2000; 68: 445-455.
    CrossrefPubMedGoogle Scholar
  • 32 Santos S.S.F., Loberto J.C.S., Martins C.A.P., Jorge A.O.C. Prevalência e sensibilidade in vitro de enterobacteriaceae e pseudomonas isoladas da cavidade bucal e bolsa periodontal de pacientes com periodontite crônica. Pós-Grad Rev 2002; 5: 74-83.
    PubMedGoogle Scholar
  • 33 Martínez A.B., Urízar J.M.A., Fenoll A.B. et al. Documento de consenso sobre el tratamiento antimicrobiano de las infecciones bacterianas odontogénicas. Med Oral Patol Oral Cir Bucal 2004; 9: 363-376.
    PubMedGoogle Scholar
  • 34 Walker C.B., Karpinia K., Baehni P. Chemotherapeutics: antibiotics and other antimicrobials. Periodontol 2000 2004; 36: 146-165.
    CrossrefPubMedGoogle Scholar
  • 35 Teixeira M.Z., Chin A.L., Martins M.A. Homeopathy and acupuncture teaching at Faculdade de Medicina da Universidade de São Paulo: the undergraduates' attitudes. Sao Paulo Med J 2005; 123: 77-82.
    CrossrefPubMedGoogle Scholar
  • 36 Widakowich J. Pharmacodynamic principles of homeopathy. Med Hypotheses 2000; 54: 721-722.
    CrossrefPubMedGoogle Scholar