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Scheuermann, J.  et al.: 2024 Science of Synthesis, 2023/5: DNA-Encoded Libraries DOI: 10.1055/sos-SD-241-00249
DNA-Encoded Libraries

4.3 Selections of DNA-Encoded Libraries to Protein Targets on Living Cells

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Buch

Herausgeber: Scheuermann, J. ; Li, Y. 

Autoren: Barluenga, S. ; Bassi, G. ; Brunschweiger, A. ; Cai, B. ; Cazzamalli, S. ; Chheda, P. ; Cui, M. ; Cui, W. ; Fang, X. ; Farrera-Soler, L. ; Favalli, N. ; Feng, J.; Foley, T. L. ; Franzini, R. M. ; Georgiev, T. ; Gillingham, D. ; Gloger, A. ; Graham, J. S. ; Granados, A. ; Heiden, S.; Hou, W. ; Huang, Y. ; Keefe, A. D. ; Krusemark, C. J. ; Li, X. ; Li, Y. ; Lin, W. ; Litovchick, A.; Liu, G. ; Lu, X. ; Lucaroni, L. ; Ma, P. ; Migliorini, F. ; Molander, G. A. ; Neri, D. ; Nie, Q. ; Oehler, S. ; Prati, L. ; Puglioli, S. ; Reddavide, F. V. ; Satz, A. L. ; Sauter, B. ; Scheuermann, J. ; Schuman, D.; Simmons, N. ; Stanway-Gordon, H. A. ; Su, W. ; Sun, J. ; Thompson, M.; Vummidi, B. R.; Wang, X. ; Wang, Y. ; Wang, Z. ; Waring, M. J. ; Willems, S.; Winssinger, N. ; Xia, B. ; Xiong, F. ; Xu, H. ; Xu, L. ; Yang, G. ; Zhang, G. ; Zhang, Y. ; Zhou, Y. 

Titel: DNA-Encoded Libraries

Print ISBN: 9783132455221; Online ISBN: 9783132437357; Buch-DOI: 10.1055/b000000342

1st edition © 2024. Thieme. All rights reserved.
Georg Thieme Verlag KG, Stuttgart

Fachgebiete: Organische Chemie;Chemische Reaktionen, Katalyse;Organometallchemie;Chemische Labormethoden, Stöchiometrie

Science of Synthesis Reference Libraries



Übergeordnete Publikation

Titel: Science of Synthesis

DOI: 10.1055/b-00000101

Reihenherausgeber: Fürstner, A. (Editor-in-Chief); Carreira, E. M.; Faul, M.; Kobayashi, S.; Koch, G.; Molander, G. A.; Nevado, C.; Trost, B. M.; You, S.-L.

Typ: Mehrbändiges Werk

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Abstract

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Membrane proteins play a crucial role in numerous physiological processes and are the most common targets of approved drugs. However, the difficulty in purifying membrane proteins has limited the application of DNA-encoded libraries (DELs) for these targets in drug discovery campaigns. In this chapter, two methodologies for the selection of DELs against cell-surface proteins directly on live cells are presented. The first method employs covalent crosslinking to capture transient interactions between DNA-linked ligands and membrane proteins, facilitating the removal of non-crosslinked molecules through stringent washing. The second approach utilizes an engineered biotin ligase enzyme tag on the target to selectively biotinylate DNA-linked ligands through induced proximity. These methods successfully address challenges such as low target-protein concentration on live cells and the low efficiency in purifying DNA–membrane-protein conjugates, offering promising tools for small-molecule discovery targeting membrane proteins.

Schlüsselwörter

DNA-encoded libraries - membrane proteins - crosslinking - biotin ligase - drug discovery - enzymatic turnover - selections