Effect of photo-activated disinfection on bond strength of three different root canal sealers

ABSTRACT

Introduction: The aim of this in vitro study was to evaluate the bond strength of Photo-Activated Disinfection (PAD) system to dentin with different root canal sealers by using a push-out test design. Materials and Methods: A total of 30 extracted mandibular premolar teeth with single and straight roots were used. The crowns were removed and the root canals were prepared by using ProTaper rotary files. The smear layer was removed and the roots were randomly divided into two groups (n = 15) according to the use of PAD system as the final disinfecting agent. Each group was then divided into 6 (n = 5) subgroups and obturated with gutta-percha and 3 different root canal sealers. The groups were Group 1: Sodium hypochlorite (NaOCl) + ethylenediaminetetraacetic acid (EDTA)-AH Plus sealer; Group 2: NaOCl + EDTA + PAD-AH Plus; Group 3: NaOCl + EDTA-Sealapex; Group 4: NaOCl + EDTA + PAD-Sealapex; Group 5: NaOCl + EDTA-mineral trioxide aggregate (MTA)-Fiallapex; and Group 6: NaOCl + EDTA + PAD-MTA-Fillapex. 1-mm thickness horizontal sections (n: 5 × 4 = 20) were sliced for the push-out bond strength measurement. Results: Group 3 and 4 showed significantly lower bond strengths compared with all the other groups (P < 0.05). No statistically significant difference was found among Groups 1, 2 and 5, but there was statistically significant difference between Group 5 and 6 (P < 0.05). Conclusion: This in vitro study indicated that the PAD system adversely affected the bond strength of the MTA Fillapex root canal sealer.

Key words:

AH Plus - bond strength - mineral trioxide aggregate Fillapex - photo-activated disinfection - Sealapex

• REFERENCES

• 1 Siqueira Jr JF, Machado AG, Silveira RM, Lopes HP, de Uzeda M. Evaluation of the effectiveness of sodium hypochlorite used with three irrigation methods in the elimination of Enterococcus faecalis from the root canal, in vitro . Int Endod J 1997; 30: 279-82
• 2 Williams JA, Pearson GJ, Colles MJ. Antibacterial action of photoactivated disinfection 1 used on endodontic bacteria in planktonic suspension and in artificial and human root canals. J Dent 2006; 34: 363-71
• 3 Souza LC, Brito PR, de Oliveira JC, Alves FR, Moreira EJ, Sampaio-Filho HR. et al. Photodynamic therapy with two different photosensitizers as a supplement to instrumentation/irrigation procedures in promoting intracanal reduction of Enterococcus faecalis . J Endod 2010; 36: 292-6
• 4 Bonsor SJ, Nichol R, Reid TM, Pearson GJ. Microbiological evaluation of photo-activated disinfection in endodontics (an in vivo study). Br Dent J 2006; 200: 337-41 329
• 5 Economides N, Kokorikos I, Kolokouris I, Panagiotis B, Gogos C. Comparative study of apical sealing ability of a new resin-based root canal sealer. J Endod 2004; 30: 403-5
• 6 Hovland EJ, Dumsha TC. Leakage evaluation in vitro of the root canal sealer cement Sealapex. Int Endod J 1985; 18: 179-82
• 7 Zmener O, Spielberg C, Lamberghini F, Rucci M. Sealing properties of a new epoxy resin-based root-canal sealer. Int Endod J 1997; 30: 332-4
• 8 Bin CV, Valera MC, Camargo SE, Rabelo SB, Silva GO, Balducci I. et al. Cytotoxicity and genotoxicity of root canal sealers based on mineral trioxide aggregate. J Endod 2012; 38: 495-500
• 9 Sleder FS, Ludlow MO, Bohacek JR. Long-term sealing ability of a calcium hydroxide sealer. J Endod 1991; 17: 541-3
• 10 Caicedo R, von Fraunhofer JA. The properties of endodontic sealer cements. J Endod 1988; 14: 527-34
• 11 Doğan H, Qalt S. Effects of chelating agents and sodium hypochlorite on mineral content of root dentin. J Endod 2001; 27: 578-80
• 12 Erickson RL. Surface interactions of dentin adhesive materials. Oper Dent 1992; (Suppl. 05) 81-94
• 13 Kataoka H, Yoshioka T, Suda H, Imai Y. Dentin bonding and sealing ability of a new root canal resin sealer. J Endod 2000; 26: 230-5
• 14 Gogos C, Stavrianos C, Kolokouris I, Papadoyannis I, Economides N. Shear bond strength of AH-26 root canal sealer to dentine using three dentine bonding agents. J Dent 2003; 31: 321-6
• 15 Lee KW, Williams MC, Camps JJ, Pashley DH. Adhesion of endodontic sealers to dentin and gutta-percha. J Endod 2002; 28: 684-8
• 16 Skidmore LJ, Berzins DW, Bahcall JK. An in vitro comparison of the intraradicular dentin bond strength of Resilon and gutta-percha. J Endod 2006; 32: 963-6
• 17 Drummond JL, Sakaguchi RL, Racean DC, Wozny J, Steinberg AD. Testing mode and surface treatment effects on dentin bonding. J Biomed Mater Res 1996; 32: 533-41
• 18 Sudsangiam S, van Noort R. Do dentin bond strength tests serve a useful purpose?. J Adhes Dent 1999; 1: 57-67
• 19 Guerisoli DM, Marchesan MA, Walmsley AD, Lumley PJ, Pecora JD. Evaluation of smear layer removal by EDTAC and sodium hypochlorite with ultrasonic agitation. Int Endod J 2002; 35: 418-21
• 20 Assmann E, Scarparo RK, Böttcher DE, Grecca FS. Dentin bond strength of two mineral trioxide aggregate-based and one epoxy resin-based sealers. J Endod 2012; 38: 219-21
• 21 Cobankara FK, Orucoglu H, Sengun A, Belli S. The quantitative evaluation of apical sealing of four endodontic sealers. J Endod 2006; 32: 66-8
• 22 Yücel AC, Güler E, Güler AU, Ertaş E. Bacterial penetration after obturation with four different root canal sealers. J Endod 2006; 32: 890-3
• 23 Vasconcelos BC, Bernardes RA, Duarte MA, Bramante CM, Moraes IG. Apical sealing of root canal fillings performed with five different endodontic sealers: Analysis by fluid filtration. J Appl Oral Sci 2011; 19: 324-8
• 24 Sagsen B, Ustün Y, Demirbuga S, Pala K. Push-out bond strength of two new calcium silicate-based endodontic sealers to root canal dentine. Int Endod J 2011; 44: 1088-91
• 25 Vivan RR, Zapata RO, Zeferino MA, Bramante CM, Bernardineli N, Garcia RB. et al. Evaluation of the physical and chemical properties of two commercial and three experimental root-end filling materials. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010; 110: 250-6
• 26 Sarkar NK, Caicedo R, Ritwik P, Moiseyeva R, Kawashima I. Physicochemical basis of the biologic properties of mineral trioxide aggregate. J Endod 2005; 31: 97-100
• 27 Camilleri J. Modification of mineral trioxide aggregate. Physical and mechanical properties. Int Endod J 2008; 41: 843-9
• 28 ØRstavik DA. Materials used for root canal obturation: Technical, biological and clinical testing. Endod Topics 2005; 12: 25-38
• 29 Wu MK, de Gee AJ, Wesselink PR. Effect of tubule orientation in the cavity wall on the seal of dental filling materials: An in vitro study. Int Endod J 1998; 31: 326-32