Radioimmuntests für die Bestimmung von Lysergsäure und einfachen Lysergsäurederivaten

Abstract

Radioimmunoassays for the quantitative and separate determination of lysergic acid and simple lysergic acid derivatives have been developed. Lysergic acid was coupled to bovine serum albumin both by the Mannich and the mixed anhydrid reaction and antibodies against these two different conjugates were raised in rabbits. ³H-lysergic acid was synthesized by basic hydrolysis of ³H-ergotamine. The antibodies produced against the conjugate, prepared by the Mannich reaction, were very specific for lysergic acid and did not cross react with any of the other ergot alkaloids tested. The antibodies raised against the conjugate, prepared by the mixed anhydrid reaction, cross reacted with all simple lysergic acid derivatives and some clavines. The antibodies exhibited high affinity towards lysergic acid and its derivatives. K = 0.46

X 10⁹ l/mol (specific antiserum), K = 3.76 × 10⁹ l/mol (polyspecific antiserum). The measuring range was between 1-12 pmol lysergic acid (use of specific antiserum) and between 0.6-31 pmol ergometrine (use of polyspecific antiserum). With the specific lysergic acid radioimmunoassay Claviceps purpurea sclerotia and saprophytic C. purpurea cultures were screened for free lysergic acid, while the ergometrine content of a saprophytic C. purpurea culture was optimized with the polyspecific lysergic acid radioimmunoassay.

Key Word Index

Claviceps purpurea - Radioimmunoassay - Lysergic Acid - Ergometrine - Screening for Lysergic Acid - Optimization of Alkaloid Content.