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Planta Med 1980; 39(6): 125-134
DOI: 10.1055/s-2008-1074913
DOI: 10.1055/s-2008-1074913
Research Articles
© Georg Thieme Verlag Stuttgart · New York
Qualitative und quantitative Analyse von Herzglykosiddrogen durch HPLC-Verfahren
1. Mitteilung: Nachweis von Bufadienoliden aus Scilla-Drogen und ZubereitungenQualitative und Quantitative HPLC-Analysis of Heart Glycosides Containing Drugs1. Communication: Determination of Bufadienolides from Scilla and Scilla-PreparationsFurther Information
Publication History
Publication Date:
29 April 2008 (online)
Abstract
The use of HPLC for qualitative determination of bufadienolides from Scilla-preparations is described. The separation systems are reversed-phase columns with methanol- or acetonitrile- water mixtures as eluents, and photometric detection at 300 nm. The method allows a fast differentiation between Scilla alba and Scilla rubra preparations and the quantitative estimation of the main glycosides, using Proscillaridin A as reference glycoside.
Key Word Index
Bufadienolides - Scilla alba L - Scilla rubra L - Scillaren A - Proscillaridin A - Scillarenin - High Performance Liquid Chromatography.