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Thromb Haemost 1960; 4(03): 365-368
DOI: 10.1055/s-0038-1654519
Originalarbeiten — Original Articles — Travaux Originaux
Schattauer GmbH

Observations on the Handling of Bovine Prothrombin and Thrombin[*]

Diethard F. Steichele
1   Department of Physiology and Pharmacology, Wayne State University, College of Medicine Detroit, Michigan, USA
,
Eberhard F. Mammen
1   Department of Physiology and Pharmacology, Wayne State University, College of Medicine Detroit, Michigan, USA
,
Yoshio Hatta
1   Department of Physiology and Pharmacology, Wayne State University, College of Medicine Detroit, Michigan, USA
,
Walter H. Seegers
1   Department of Physiology and Pharmacology, Wayne State University, College of Medicine Detroit, Michigan, USA
› Author Affiliations
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Publication History

Publication Date:
17 June 2018 (online)

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Summary

In the handling of thrombin activity is lost by freeze drying, and by dialysis of dilute solutions. Acetone drying can be done without loss of activity. Acetylated thrombin does not lose activity during freeze drying. Prothrombin activity is retained during freeze drying and during dialysis, but is lost with acetone drying and when dilute solutions are placed in ordinary glassware that is not lined with paraffin.

* This investigation was supported by a research grant H-2026 from the National Heart Institute, National Institutes of Health. Funds for research fellowships in physiology were provided by Parke, Davis and Company.


 

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