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Thromb Haemost 1960; 4(03): 323-330
DOI: 10.1055/s-0038-1654513
Originalarbeiten — Original Articles — Travaux Originaux
Schattauer GmbH

A Rapid Quantitative Assay of Factor VIII (AHG) Without the Use of Haemophilia A Plasma

Ernst G. Jung
1   Coagulation Laboratory (Prof. F. Koller) of the Department of Medicine (Prof. P. H. Rossier) University of Zurich, Switzerland
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Publication History

Publication Date:
17 June 2018 (online)

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Summary

A new method for the quantitative determination of factor VIII (AHG) without using haemophilia A plasma as test substrate has been described. The comparison to Geiger et al. shows a good concordance. The normal range of factor VIII in adults is 50—150% (normal plasma taken as 100%) and the average value of healthy newborns is 78%. This is compared to the average values of the other clotting factors in newborns. Factor VIII is not affected by deficient vitamin K resorption.

 

  • References

  • (1) Aggeler P. M, White S. G, Gelndening M. B, Page E. D, Leake T. B, Bates G. Plasma thromboplastin component (PTC) deficiency: a new disease resembling haemophilia. Proc. Soc. exp. Biol. (N. Y.) 79: 692 1952;
    CrossrefPubMedGoogle Scholar
  • (2) Bachmann F, Duckert F, Flückiger P, Hitzig W, Koller F. Über einen neuartigen kongenitalen Gerinnungsdefekt (Mangel an Stuartfaktor). Thromb. Diath. haem. 1: 87 1957;
    PubMedGoogle Scholar
  • (3) Biggs R, Douglas A. S, Macfarlane R. G, Dacie J. V, Pitney W. R, Merskey C, O’Brien J. F. Christmas disease, a condition previously mistaken for haemophilia. Brit. med. J. 27: 1378 1952;
    PubMedGoogle Scholar
  • (4) Biggs R, Douglas A. S. The thromboplastin generation test. J. clin. Pathol. 6: 23 1953;
    CrossrefPubMedGoogle Scholar
  • (5) Biggs R, Macfarlane R. G. Human blood coagulation. 2nd Ed.. Blackwell; Oxford: 1957
    Google Scholar
  • (6) Biggs R, Macfarlane R. G. Haemophilia and related conditions: a survey of 187 cases. Brit. J. Haemat. 4: 1 1958;
    CrossrefPubMedGoogle Scholar
  • (7) Bounameaux Y. Dosage du facteur VIII en un temps. Acta haemat. 17: 355 1957;
    CrossrefPubMedGoogle Scholar
  • (8) Brinkhous K. M, Langdell R. D, Penick G. D, Graham J. B, Wagner R. H. Newer approches to the study of haemophilia and haemophiloid states. J. Amer. med. Ass. 154: 481 1954;
    CrossrefPubMedGoogle Scholar
  • (9) Brinkhous K. M. Haemophilia. Bull. N. Y. Acad. Med. 3: 325 1954;
    PubMedGoogle Scholar
  • (10) Cramer R, Matter M, Loeliger A. Die Hämophilie B. Zwei familiäre Fälle der Bluterkrankheit bedingt durch einen Mangel eines neuen Gerinnungsfaktors (Christmas disease). Helv. paediat. Acta 8: 185 1953;
    PubMedGoogle Scholar
  • (11) Cramer R, Flückiger P, Gasser C, Koller F, Loeliger A, Matter M. Hämophilie B. Acta haemat. 10: 65 1953;
    CrossrefPubMedGoogle Scholar
  • (12) van Crefeld S. Haemophilia and allied diseases. Rocky Mount. med. J.. march 1958
    PubMedGoogle Scholar
  • (13) Deutsch E. Verschiedene Formen der Hämophilie. Thromb. Diath. haem. 1: 93 1957;
    PubMedGoogle Scholar
  • (14) Duckert F, Flückiger P, Isenschmid H, Matter M, Vogel-Meng Koller F. A modification of the thromboplastin generation test. Acta haemat. 12: 197 1954;
    CrossrefPubMedGoogle Scholar
  • (15) Duckert F. Le diagnostic des coagulopathies. 8 Coll. Biol. clin.. Bruxelles: presse acad. europ.; 635 1958
    Google Scholar
  • (16) Fantl P, Sawers R. J. A syndrome under review. Austr. Ann. Med. 3: 245 1954;
    PubMedGoogle Scholar
  • (17) Fisch U, Duckert F. Some aspects of kinetics of the first stages of blood thromboplastin formation. Thromb. Diath. haem. 3: 98 1958;
    PubMedGoogle Scholar
  • (18) Frick P. G. The relative incidence of antihaemophilic globulin (AHG), PTC and PTA deficiency. J. Lab. clin. Med. 43: 860 1954;
    PubMedGoogle Scholar
  • (19) Geiger M, Duckert F, Koller F. Quantitative Bestimmung von Faktor VIII und IX bei Blutersippen. Kongr. europ. Ges. Haemat.. 413 Springer; Heidelberg: 1956
    Google Scholar
  • (20) Graham J. B, MacLendon W. W, Brinkhous K. M. Mild haemophilia, an allelic form of the disease. Brit. med. J. 2: 97 1953;
    PubMedGoogle Scholar
  • (21) Hougie C, Graham J. B. The blood clotting role and mode of inheritance of the Stuart factor. 6th Congr. int. Soc. Bl. Transf.. 80 Boston: 1956
    Google Scholar
  • (22) Hougie C. Studies on the fate of coagulation factors during clotting of normal and pathological blood. Thromb. Diath. haem. 3: 578 1959;
    PubMedGoogle Scholar
  • (23) Koller F, Krüsi G, Luchsinger P. Über eine hämophilieartige hereditäre hämorrhagische Diathese mit Störung der ersten Gerinnungsphase. Arch. Jul. Klaus-Stiftg. 25: 16 1950;
    PubMedGoogle Scholar
  • (24) Koller F, Krüsi G, Luchsinger P. Über eine besondere Form der hämorraghischen Diathese. Schweiz. med. Wschr. 80: 1101 1950;
    PubMedGoogle Scholar
  • (25) Koller F, Loeliger A, Duckert F. Experiments on a new clotting factor (factor VII). Acta haemat. 6: 1 1951;
    CrossrefPubMedGoogle Scholar
  • (26) Koller F. Zur Differenzierung der Hämophilie. Arch. Jul. Klaus-Stiftg. 28: 254 1953;
    PubMedGoogle Scholar
  • (27) Koller F. Das Verhalten der Gerinnungsfaktoren in vitro und in vivo. Proc. 7th Congr. int. Soc. Bl. Transf.. 731 Rome: 1958
    Google Scholar
  • (28) Langdell R. D, Wagner R. H, Brinkhous K. M. Effect of antihaemophilic factor on one-stage clotting tests. A presumptive test for haemophilia and a simple one-stage antihaemophilic assay procedure. J. Lab. clin. Med. 41: 637 1953;
    PubMedGoogle Scholar
  • (29) Lewis J. H, Ferguson J. H, Fresh J. W, Zucker M. B. Primary haemorrhagic diseases. J. Lab. clin. Med. 49: 211 1957;
    PubMedGoogle Scholar
  • (30) Loeliger A, Koller F. Behaviour of factor VII and prothrombin in late pregnancy and in the newborn. Acta haemat. 7: 157 1952;
    CrossrefPubMedGoogle Scholar
  • (31) Marx R. Über Hämophilie und Pseudohämophilie. Münch. med. Wschr. 101 (881) 924 1959;
    PubMedGoogle Scholar
  • (32) Moor-Jankowski J. K, Huser H. J, Rosin S, Truog G, Schneeberger M, Geiger M. Hämophilie B. Genetische, klinische und gerinnungsphysiologische Aspekte. S. Karger; Basel: 1958
    Google Scholar
  • (33) den Ottolander G. H, Hoorweg P. G. De frequentie van haemophilie A en B. Ned. T. Geneesk. 99: 2037 1955;
    PubMedGoogle Scholar
  • (34) Owren P. The coagulation of blood. Investigation on a new clotting factor. Acta med. scand.. 194. Suppl. 1947
    PubMedGoogle Scholar
  • (35) Pavlovsky A. Haemophilia: Comparative study of the varieties A and B. 6th Congr. int. Soc. Haemat.. 415 Boston: 1956
    Google Scholar
  • (36) Pitney W. R, Arnold B. J. Plasma antihaemophilic factor (AHF) concentrations in families of patients with haemorrhagic states. Brit. J. Haemat. 5: 184 1959;
    CrossrefPubMedGoogle Scholar
  • (37) Pool J. G, Robinson J. Observation on Plasma banking and transfusion procedures for haemophilie patients using a quantitative assay for AHG. Brit. J. Haemat. 5: 24 1959;
    CrossrefPubMedGoogle Scholar
  • (38) Quick A. J. The prothrombin in haemophilia and in obstructive jaundice. J. biol. Chem. 109: 523 1935;
    PubMedGoogle Scholar
  • (39) Rappaport S. I. Survey of large haemophilie population. Congr. int. Soc. Haemat.. Boston: 1956
    Google Scholar
  • (40) Ratnoff O. D, Margolis A. On the epidemiology of haemophilia and Christmas disease. New Engl. J. Med. 256: 845 1957;
    CrossrefPubMedGoogle Scholar
  • (41) Remde W. Thrombozytenfunktionsstörungen bei Hämophiliekranken. Transact. 6th Congr. europ. Soc. Haemat.. 579 Kopenhagen: 1957
    Google Scholar
  • (42) Rosenthal R. L, Dreskin O. H, Rosenthal N. New haemophilia-like disease caused by deficiency of a third plasma thromboplastin factor. Proc. Soc. exp. Biol. (N.Y.) 82: 171 1953;
    CrossrefPubMedGoogle Scholar
  • (43) Rosenthal R. L. Haemophilia and haemophilia-like diseases caused by deficiencies in plasma thromboplastin factors. Amer. J. Med. 17: 57 1954;
    CrossrefPubMedGoogle Scholar
  • (44) Soulier J. B, Larrieu M. M. Nouvelle méthode du diagnostic de l’hémophilie. Dosage des facteurs antihémophiliques A et B. Sang 24: 205 1953;
    PubMedGoogle Scholar
  • (45) Soulier J. P. Formes cliniques de l’hémophilie. Rev. Prat. (Paris) 6: 2295 1956;
    PubMedGoogle Scholar
  • (46) Verstraete M, Vanderbrouke J. Deficient thromboplastin formation in man. Amer. J. Med. 22: 624 1957;
    CrossrefPubMedGoogle Scholar
  • (47) Waaler B. A. A simple one-stage method for the assay of antihemophilic factor A. Scand. J. clin. Lab. Invest. 11: 194 1959;
    CrossrefPubMedGoogle Scholar
  • (48) Wolf P. Studies of temperature and pH stability of human antihaemophilic factor (AHF) in plasma and in a concentrate. Brit. J. Haemat. 5: 169 1959;
    CrossrefPubMedGoogle Scholar