In vitro Anti-Influenza Viral Activities of Constituents from Caesalpinia sappan

Abstract

Six constituents with neuraminidase (NA) inhibitory activity, namely brazilein, brazilin, protosappanin A, 3-deoxysappanchalcone, sappanchalcone and rhamnetin, were isolated from the hearthwood of Caesalpinia sappan (Leguminosae). Their in vitro anti-influenza virus activities were evaluated with the cytopathic effect (CPE) reduction method. The results showed that 3-deoxysappanchalcone and sappanchalcone exhibited the highest activity against influenza virus (H3N2) with IC₅₀ values of 1.06 and 2.06 μg/mL, respectively, in comparison to the positive control oseltamivir acid and ribavirin with IC₅₀ values of 0.065 and 9.17 μg/mL, respectively.

Abbreviations

CPE:cytopathic effect

NA:neuraminidase

Key words

Caesalpinia sappan - Leguminosae - influenza virus - neuraminidase - sappanchalcone

References

• 1 Liu A, Wang Y, Du G. Neuraminidase as a target for drugs for the treatment of influenza.  Drugs Future. 2005;  30 799-806
• 2 Shu S H, Zhang L, Du G H, Qing H L. Study on the chemical constituents of Caesalpinia sappan. .  Nat Prod Res Dev. 2007;  19 63-6
• 3 Nagai M, Nagumo S, Lee S M, Eguchi I, Kawai K I. Protosappanin A, a novel biphenyl compound from Sappan Lignum.  Chem Pharm Bull. 1986;  34 1-6
• 4 Namikoshi M, Nakata H, Nuno M, Ozawa T, Saitoh T. Homoisoflavonoids and related compounds III. Phenolic constituents of Caesalpinia japonica Sieb. et Zucc.  Chem Pharm Bull. 1987;  35 3568-75
• 5 Sharma S K, Parmar V S. Novel constituents of Tamarix species.  J Sci Industrial Res. 1998;  57 873-90