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Planta Med 2023; 89(05): 561-570
DOI: 10.1055/a-1984-8515
Pharmacokinetic Investigations
Original Papers

Comparative Pharmacokinetic Investigation on Multiple Active Aminoalcohol-Diterpenoid Alkaloids after Single Oral Administrations of Monomers and Aqueous Extract of Fuzi (Aconiti Lateralis Radix Praeparata) by UFLC-MS/MS

Ming-Hai Tang
1   West China School of Pharmacy, Sichuan University, Chengdu, China
2   State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China
,
Fu-Li Peng
1   West China School of Pharmacy, Sichuan University, Chengdu, China
,
Xiu-Xiu Liu
1   West China School of Pharmacy, Sichuan University, Chengdu, China
,
Ruo-Bing Chao
1   West China School of Pharmacy, Sichuan University, Chengdu, China
› Author AffiliationsSupported by: National Natural Science Foundation of China 81803705
Supported by: Sichuan Province Science and Technology Program 2020YFS0067
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Abstract

To further study the aminoalcohol-diterpenoid alkaloids (ADAs) in Fuzi (Aconiti Lateralis Radix Praeparata), a simple and sensitive UFLC-MS/MS method was established and validated for the determination of five ADAs, aconine, mesaconine, hypaconine, deoxyaconine and fuziline, in rat plasma to compare the pharmacokinetic characteristics of pure ADAs and Fuzi decoction. After precipitating protein with methanol, plasma samples were isolated at 0.5 mL/min flow rate on Waters Acquity UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 µm). The mobile phase was composed of 0.1% formic acid-water and methanol with gradient elution. Mass spectrometric inspection was conducted on a 5500 UFLC-MS/MS system with an electrospray ionization source in patterns of positive ion and multiple reaction-monitoring (MRM). All calibration curves were proved to have acceptable linearity (r2 > 0.99) in linear ranges. Intra-day and inter-day precision and the accuracy met the requirements. The matrix effects of all analytes were between 85% and 115% of three concentration levels. This method has been under verification for comparative pharmacokinetic research after oral administration between aqueous extract of Fuzi and single pure ADAs. The results demonstrated that there are evident pharmacokinetic discrepancies between them, and administration in the extract form instead of pure form may contribute to higher absorption.

Key words

aminoalcohol-diterpenoid alkaloids - pharmacokinetics - aqueous extract of Fuzi - UFLC-MS/MS - Aconitum carmichaelii - Ranunculaceae


Publication History

Received: 12 December 2021

Accepted after revision: 13 September 2022

Article published online:
23 January 2023

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